推荐产品
用途
sufficient for 100 fluorometric tests
应用
pharmaceutical
检测方法
fluorometric
相关疾病
cardiovascular diseases
储存温度
2-8°C
一般描述
The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol forming cholesteryl ester. This activity occurs on the surface of high density lipoprotein (HDL) and the cholesteryl esters formed by LCAT are incorporated into the core of HDL.
特点和优势
- Non-enzymatic determination of cholesteryl ester formation.
- Not affected by LCAT inhibitors or other chemical compounds.
- Dilution or non-dilution type assay.
- Assay components stable for up to one year.
- Compatible with high-throughput handling systems.
适用性
Suitable for high-throughput screening, mechanism of action studies and structureactivity relationship (SAR) work of LCAT in plasma and serum
原理
The LCFC-LCAT Acyltransferase Activity Assay is a fluorometric assay useful for measuring the acyltransferase activity of LCAT in serum or plasma. The method detects changes in LCAT free cholesterol (LCFC) levels in the sample without the use of cholesterol oxidase, peroxidase, or the generation of hydrogen peroxide. Detection is not affected by iodoacetate or other LCAT inhibitors. LCAT activity results in a fluorometric (λEx = 320 nm/λEm = 405 nm) product proportional to the amount of free cholesterol present.
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Deficient cholesterol esterification in plasma of apoc2 knockout zebrafish and familial chylomicronemia patients.
Liu C, et al.
PLoS ONE, 12(1), e0169939-e0169939 (2017)
Chao Liu et al.
PloS one, 12(1), e0169939-e0169939 (2017-01-21)
Hypertriglyceridemia is an independent risk factor for cardiovascular disease. Apolipoprotein C-II (APOC2) is an obligatory cofactor for lipoprotein lipase (LPL), the major enzyme catalyzing plasma triglyceride hydrolysis. We have created an apoc2 knockout zebrafish model, which mimics the familial chylomicronemia
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门