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Merck
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安全信息

MAK306

Sigma-Aldrich

LCFC-LCAT 脂肪酰转移酶活性检测试剂盒

Supplied by Roar Biomedical, Inc., sufficient for 100 fluorometric tests

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UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 100 fluorometric tests

应用

pharmaceutical

检测方法

fluorometric

相关疾病

cardiovascular diseases

储存温度

2-8°C

相关类别

一般描述

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol forming cholesteryl ester. This activity occurs on the surface of high density lipoprotein (HDL) and the cholesteryl esters formed by LCAT are incorporated into the core of HDL.

特点和优势

  • Non-enzymatic determination of cholesteryl ester formation.
  • Not affected by LCAT inhibitors or other chemical compounds.
  • Dilution or non-dilution type assay.
  • Assay components stable for up to one year.
  • Compatible with high-throughput handling systems.

适用性

Suitable for high-throughput screening, mechanism of action studies and structureactivity relationship (SAR) work of LCAT in plasma and serum

原理

The LCFC-LCAT Acyltransferase Activity Assay is a fluorometric assay useful for measuring the acyltransferase activity of LCAT in serum or plasma. The method detects changes in LCAT free cholesterol (LCFC) levels in the sample without the use of cholesterol oxidase, peroxidase, or the generation of hydrogen peroxide. Detection is not affected by iodoacetate or other LCAT inhibitors. LCAT activity results in a fluorometric (λEx = 320 nm/λEm = 405 nm) product proportional to the amount of free cholesterol present.

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Deficient cholesterol esterification in plasma of apoc2 knockout zebrafish and familial chylomicronemia patients.
Liu C, et al.
PLoS ONE, 12(1), e0169939-e0169939 (2017)
Chao Liu et al.
PloS one, 12(1), e0169939-e0169939 (2017-01-21)
Hypertriglyceridemia is an independent risk factor for cardiovascular disease. Apolipoprotein C-II (APOC2) is an obligatory cofactor for lipoprotein lipase (LPL), the major enzyme catalyzing plasma triglyceride hydrolysis. We have created an apoc2 knockout zebrafish model, which mimics the familial chylomicronemia

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