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检测方法
colorimetric
相关疾病
cancer
储存温度
−20°C
一般描述
GAPDH（甘油醛-3-磷酸脱氢酶；EC 1.2.1.12）可催化甘油醛-3-磷酸酯（GAP）转化为1,3-二磷酸甘油酸盐（BPG），对糖酵解具有重要作用。该酶参与了细胞凋亡、膜转运、铁代谢和核移位等细胞过程。GAPDH（管家基因）表达是稳定的组成型表达。GAPDH活性异常与细胞增殖异常和癌变相关。GAPDH活性的准确定量对于疾病诊断和研究正常细胞生理学非常重要。
应用
GAPDH 活性检测试剂盒已用于测量睾丸中甘油醛-3-磷酸脱氢酶的酶活性。
特点和优势
兼容高通量操作系统。
适用性
适用于各种组织和细胞培养样本。
原理
GAPDH 活性检测试剂盒为监测各种样品中的 GAPDH 活性提供了一种简单而灵敏的方法。GAPDH 的活性是通过偶联酶反应测定的，其中 GAP 被 GAPDH 转化为 BPG。这导致比色（450nm）产物与存在的酶活性成比例。该检测对 100 mUnites/mL 敏感。一个单位的 GAPDH 活性是指在 pH7.2，37℃ 下，每分钟产生 1.0 mmole NADH 的酶量。
警示用语：
Danger
危险声明
危险分类
Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
储存分类代码
8A - Combustible corrosive hazardous materials
法规信息
常规特殊物品
历史批次信息供参考:
Samir El Qaidi et al.
Scientific reports, 11(1), 3834-3834 (2021-02-17)
Type III secretion system effector proteins have primarily been characterized for their interactions with host cell proteins and their ability to disrupt host signaling pathways. We are testing the hypothesis that some effectors are active within the bacterium, where they
Altered expression profile of glycolytic enzymes during testicular ischemia reperfusion injury is associated with the p53/TIGAR pathway: effect of fructose 1, 6-diphosphate.
May A L and Renno W M
PeerJ, 4, e2195-e2195 (2016)
May Al-Maghrebi et al.
PeerJ, 4, e2195-e2195 (2016-07-22)
Background. Testicular ischemia reperfusion injury (tIRI) is considered the mechanism underlying the pathology of testicular torsion and detorsion. Left untreated, tIRI can induce testis dysfunction, damage to spermatogenesis and possible infertility. In this study, we aimed to assess the activities
Catríona M Dowling et al.
Science advances, 7(3) (2021-02-02)
Triple-negative breast cancer (TNBC) is a subtype of breast cancer without a targeted form of therapy. Unfortunately, up to 70% of patients with TNBC develop resistance to treatment. A known contributor to chemoresistance is dysfunctional mitochondrial apoptosis signaling. We set
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