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检测方法
fluorometric
相关疾病
endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases
储存温度
−70°C
一般描述
钙蛋白酶激活涉及多种形式的生理和病理过程(如细胞凋亡)。钙蛋白酶活化需要细胞膜和Ca2+,而活化的钙蛋白酶则会释放到细胞质中。钙蛋白酶活性检测试剂盒包含优化的缓冲液和试剂,为钙蛋白酶活性测定提供了一种便捷方法。
适用性
适用于分析凋亡和其他样本中的钙蛋白酶活性。
原理
钙蛋白酶活性荧光测定试剂盒基于钙蛋白酶底物 Ac-LLY-AFC 的裂解。Ac-LLY-AFC 衬底发射蓝光(最大值 = 400 nm);经钙蛋白酶裂解后,游离 AFC 发射黄绿色荧光 (max = 505 nm),可使用荧光计或荧光酶标仪定量。通过比较处理样品和正常对照样品的荧光强度,可以确定钙蛋白酶活性的变化。
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产品编号
说明
价格
警示用语:
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
10 - Combustible liquids
法规信息
常规特殊物品
Judy Kim et al.
Bioscience reports, 40(1) (2020-01-09)
A key mechanism mediating cellular adaptive responses to hypoxia involves the activity of hypoxia-inducible factor 1 (HIF-1), a transcription factor composed of HIF-1α, and HIF-1β subunits. The classical mechanism of regulation of HIF-1 activity involves destabilisation of HIF-1α via oxygen-dependent
Huilin Cheng et al.
Food science of animal resources, 42(2), 266-279 (2022-03-22)
This study was conducted to evaluate the proteolysis trends and change in meat quality during 10 days of cold storage in duck M. pectoralis major (PM) and M. iliotibialis (IL). Duck IL had a higher pH and greater degree of
Alexander Otahal et al.
Scientific reports, 10(1), 959-959 (2020-01-24)
Hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors (statins) have been shown to overcome tyrosine kinase inhibitor (TKI) resistance in epithelial growth factor receptor (EGFR) mutated non-small cell lung cancer (NSCLC) cells in vivo and in vitro. However, little is known about the
Huilin Cheng et al.
Poultry science, 101(12), 102185-102185 (2022-10-12)
The proteolysis trends and meat quality of the chicken pectoralis major (PM) and iliotibialis (IL) muscles stored at 4°C for 7 d were investigated. After 7 d of storage, the purge loss was higher (P < 0.05) in PM than
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