推荐产品
检测方法
fluorometric
储存温度
−20°C
一般描述
烟酰胺二核苷酸（NADPH）的还原形式可充当许多氧化还原酶的辅助因子，并有助于还原性生物合成途径。NADPH主要由葡萄糖通过氧化戊糖磷酸途径产生。
应用
高灵敏度NADPH分析试剂盒已被用于测定细胞内NAD（P）H和NAD（P）+ 水平。
适用性
适于检测多种生物样品中的NADPH，包括细胞及组织培养上清液和纯化线粒体等。
原理
高灵敏度NADPH定量荧光分析试剂盒适用于定量组织、细胞和线粒体中的NADPH（范围为2-10 pmole/孔）。该试剂盒可在酶循环反应中检测细胞内的NAPDH，而不需要从样品中对NADPH进行纯化。其通过与NADPH的量成正比的荧光产物 (λex = 535/ λem = 587 nm) 对NADPH进行测定。
仅试剂盒组分
产品编号
说明
- NADPH Extraction Buffer
- NADPH Cycling Buffer
- High-Sensitivity Probe
- NADPH Cycling Enzyme Mix
- NADPH Standard
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