推荐产品
检测方法
colorimetric
相关疾病
neurological disorders
储存温度
−20°C
基因信息
human ... SDHA(6389) , SDHAF1(644096) , SDHAF2(54949) , SDHB(6390) , SDHC(6391) , SDHD(6392)
mouse ... SDHA(66945) , SDHAF1(68332) , SDHAF2(66072) , SDHB(67680) , SDHC(66052) , SDHD(66925)
rat ... SDHA(157074) , SDHAF2(361726) , SDHB(298596) , SDHC(289217) , SDHD(363061)
一般描述
琥珀酸脱氢酶（SDH；酶代码 EC 1.3.5.1）是一种线粒体酶，在真核生物和细菌中催化琥珀酸氧化为延胡索酸，并携带电子从 FADH 到 CoQ。在通过三羧酸循环和电子传递链维持细胞能量代谢中起中心作用。SDH 突变引起遗传性副神经节瘤/嗜铬细胞瘤综合征和一种被称为 Leigh 综合征的神经退行性疾病。
应用
琥珀酸脱氢酶活性比色测定试剂盒已用于测定样品中琥珀酸脱氢酶的酶活性。
适用性
本试剂盒适于检测不同生物样品（如细胞、组织培养上清液和纯化的线粒体）中琥珀酰脱氢酶的活性。
原理
琥珀酸脱氢酶活性比色检测试剂盒为测定多种组织、细胞和分离线粒体中的 SDH 活性提供了一种简单、灵敏的程序。通过产生与存在的酶活性成比例的 600nm 吸光度的产物来确定 SDH 活性。一个单位的 SDH 是在 pH7.2，25℃ 下每分钟产生 1.0μmole DCIP 的酶量。
仅试剂盒组分
产品编号
说明
- SDH Assay Buffer
- SDH Substrate Mix
- SDH Probe
- DCIP Standard, 2 mM
- SDH Positive Control
WGK
WGK 3
法规信息
常规特殊物品
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