检测方法
colorimetric
相关疾病
neurological disorders
储存温度
−20°C
基因信息
human ... MDH1(4190) , MDH1B(130752) , MDH2(4191)
mouse ... MDH1(17449) , MDH1B(76668) , MDH2(17448)
rat ... MDH1(24551) , MDH1B(316444) , MDH2(81829)
一般描述
苹果酸脱氢酶（MDH; EC 1.1.1.37）是一种NAD氧化还原酶，其可催化苹果酸盐和草酸乙酯的NAD/NADH依赖性相互转化。该反应对于维持苹果酸/天冬氨酸穿梭线粒体膜和线粒体基质中的Kerbs三羧酸循环来说至关重要。研究人员已在诸如阿尔茨海默氏病′的神经变性疾病中观察到MDH活性的增加。
应用
苹果酸脱氢酶测定试剂盒已被用于LDH（乳酸脱氢酶）和苹果酸脱氢酶（MDH1）的测定。 它也被用于估测异柠檬酸脱氢酶 和琥珀酸脱氢酶的活性。
适用性
适于测定不同生物样品内的苹果酸脱氢酶活性，如细胞和组织培养上清液以及纯化所得的线粒体。
原理
苹果酸脱氢酶检测试剂盒提供了一种简单灵敏的方法，用于测定各种组织、细胞培养物和分离的线粒体中的MDH活性。通过产生与酶活性成比例的在450nm处有吸收的产物来确定MDH的活性。在37℃，pH 9.5条件下，一个单位MDH为每分钟产生1.0 μmol NADH所需的酶量。
仅试剂盒组分
产品编号
说明
- MDH Assay Buffer
- MDH Substrate
- MDH Enzyme Mix
- MDH Developer
- NADH Standard
- MDH Positive Control
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Skin Corr. 1B
储存分类代码
8A - Combustible corrosive hazardous materials
法规信息
含少量动物源组分生物产品
Jinliang Wang et al.
Scientific reports, 9(1), 12300-12300 (2019-08-25)
The human mitochondrial heat shock protein 60 (hsp60) is a tetradecameric chaperonin that folds proteins in the mitochondrial matrix. An hsp60 D3G mutation leads to MitCHAP-60, an early onset neurodegenerative disease while hsp60 V72I has been linked to SPG13, a
Two plant-derived aporphinoid alkaloids exert their antifungal activity by disrupting mitochondrial iron-sulfur cluster biosynthesis.
Tripathi S K, et al.
The Journal of Biological Chemistry, jbc-M117 (2017)
Identification of a small molecule inhibitor of 3-phosphoglycerate dehydrogenase to target serine biosynthesis in cancers.
Mullarky E, et al.
Proceedings of the National Academy of Sciences of the USA, 113(7), 1778-1783 (2016)
Nan Nan et al.
Plant biotechnology journal, 18(1), 172-184 (2019-06-05)
Salinity is an important environmental factor that adversely impacts crop growth and productivity. Malate dehydrogenases (MDHs) catalyse the reversible interconversion of malate and oxaloacetate using NAD(H)/NADP(H) as a cofactor and regulate plant development and abiotic stress tolerance. Vitamin B6 functions
Edouard Mullarky et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(7), 1778-1783 (2016-02-03)
Cancer cells reprogram their metabolism to promote growth and proliferation. The genetic evidence pointing to the importance of the amino acid serine in tumorigenesis is striking. The gene encoding the enzyme 3-phosphoglycerate dehydrogenase (PHGDH), which catalyzes the first committed step
实验方案
Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.
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