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检测方法
colorimetric
fluorometric
相关疾病
endocrinological disorders, diabetes; obesity
储存温度
−20°C
一般描述
Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes.
适用性
该试剂盒适用于从组织中分离脂肪细胞和脂解作用的测定。
原理
The Lipolysis (Adipocyte) Kit provides contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent (λex = 535/ λem = 587 nm) or colorimetric (570 nm) product proportional to the amount of glycerol present.
仅试剂盒组分
产品编号
说明
- Collagenase (0.2%)
- Collagenase Stop Buffer
- Adipocyte Wash Buffer
- Adipocyte Lipolysis Buffer
- Glycerol Assay Buffer
- Glycerol Probe, in DMSO
- Glycerol Enzyme Mix
- Glycerol Standard, 100 mM
- Isoproterenol, 10 mM
- Cell Strainer
查看所有结果 (10)
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 3
法规信息
新产品
José M Bravo-San Pedro et al.
Cell metabolism, 30(4), 754-767 (2019-08-20)
Autophagy facilitates the adaptation to nutritional stress. Here, we show that short-term starvation of cultured cells or mice caused the autophagy-dependent cellular release of acyl-CoA-binding protein (ACBP, also known as diazepam-binding inhibitor, DBI) and consequent ACBP-mediated feedback inhibition of autophagy.
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