检测方法
colorimetric
相关疾病
endocrinological disorders, diabetes; cancer
储存温度
−20°C
基因信息
human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)
一般描述
柠檬酸合酶（CS；EC 2.3.3.1）是一种线粒体酶，存在于所有能够进行氧化代谢的细胞中。它在Krebs 三羧酸循环中，催化草酰乙酸合成柠檬酸盐。1,2柠檬酸合酶参与能量产生、脂肪生成和胆固醇生成，其活性遵循昼夜节律模式。
应用
柠檬酸合酶活性检测试剂盒已用于测量样品中柠檬酸合酶的活性。
适用性
适于测定不同生物样品内的柠檬酸合成酶活性，如细胞和组织培养上清液以及纯化所得的线粒体。
原理
柠檬酸合酶活性检测试剂盒提供了一种简单直接的方法，用于测量各种组织、细胞培养物（粘附或悬浮）和纯化线粒体中柠檬酸合酶的活性。使用偶联酶反应测定柠檬酸合酶活性，其产生与存在的酶活性成比例的比色（412 nm）产物。一个单位的柠檬酸合酶是在25 °C和pH 7.2 下，每分钟产生 1.0 mmole CoA的酶的量。
仅试剂盒组分
产品编号
说明
- CS Assay Buffer
- CS Substrate Mix
- CS Developer
- GSH Standard, Reduced
- CS Positive Control
警示用语：
Danger
危险声明
危险分类
Eye Irrit. 2 - Resp. Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 3
法规信息
动植物源性产品
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