推荐产品
检测方法
colorimetric
相关疾病
endocrinological disorders, diabetes; orthopedic diseases; neurological disorders; rheumatological diseases; cancer; cardiovascular diseases
储存温度
−20°C
基因信息
human ... OGDH(4967)
mouse ... OGDH(18293)
rat ... OGDH(360975)
一般描述
α-酮戊二酸脱氢酶（α-KGDH，EC 1.2.4.2）是一种高度调节的Krebs循环酶，可形成线粒体2-氧戊二酸脱氢酶复合物的E1亚基。它可催化α-酮戊二酸转化为琥珀酰-CoA，并产生直接为呼吸链提供电子的NADH。降低的α-KGDH复合物活性与氧化应激和神经退行性疾病如阿尔茨海默氏症和帕金森病有关。
应用
α-酮戊二酸脱氢酶活性比色测定试剂盒已用于测定样品中α-酮戊二酸脱氢酶的酶活性。
适用性
适于测定多种样品中的α-酮戊二酸脱氢酶，如组织、细胞核纯化所得的线粒体。
原理
α-酮戊二酸脱氢酶活性比色测定试剂盒提供了对各种组织、细胞和分离的线粒体中α-酮戊二酸脱氢酶活性进行简单测定的方法。通过测量比色产物对α-KGDH活性进行测定，该比色产物在450nm处的吸光度（A450）与存在的酶活性成比例。在pH7.5,37℃条件下，一个单位α-酮戊二酸脱氢酶为每分钟产生1.0 μmole NADH所需的酶量。
仅试剂盒组分
产品编号
说明
- KGDH Assay Buffer
- KGDH Substrate
- KGDH Developer
- NADH Standard
- KGDH Positive Control
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
储存分类代码
8A - Combustible corrosive hazardous materials
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Poldip2 is an oxygen-sensitive protein that controls PDH and αKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer.
Paredes F, et al.
Proceedings of the National Academy of Sciences of the USA, 115(38), 201720693-201720693 (2018)
Alpha-ketoglutarate dehydrogenase: a target and generator of oxidative stress.
Tretter L and Vera A
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 360(1464), 2335-2345 (2005)
Sirtuin lipoamidase activity is conserved in bacteria as a regulator of metabolic enzyme complexes.
Rowland E A, et al.
mBio, 8(5), e01096-e01017 (2017)
Poldip2 is an oxygen-sensitive protein that controls PDH and αKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer.
Paredes F, et al.
Proceedings of the National Academy of Sciences of the USA, 2018, 201720693-201720693 null
Alexandre Buffet et al.
The Journal of clinical endocrinology and metabolism, 106(2), 459-471 (2020-11-13)
Pheochromocytomas and paragangliomas (PPGLs) are neuroendocrine tumors in which altered central metabolism appears to be a major driver of tumorigenesis, and many PPGL genes encode proteins involved in the tricarboxylic acid (TCA) cycle. While about 40% of PPGL cases carry
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