用途
sufficient for 100 fluorometric tests
检测方法
fluorometric
相关疾病
endocrinological disorders, diabetes; neurological disorders; cancer
储存温度
−20°C
一般描述
蛋白质降解最常通过两种主要机制发生，溶酶体降解和蛋白酶体介导的降解。溶酶体降解是通过蛋白水解酶活性发生的，且几乎无特异性。蛋白酶体降解是通过靶蛋白的泛素化而针对特定蛋白进行的。靶蛋白通常是多泛素化的，并可激活 26S 蛋白酶体进行蛋白水解。蛋白酶体 26S 是蛋白酶体最常见的形式，是一种 ATP 依赖性蛋白水解复合物，含有一个 20S (700-kDa) 核心颗粒结构和两个 19S (700-kDa) 调控帽。
蛋白酶体负责蛋白质的回收利用，维持蛋白质合成与降解的平衡。蛋白酶体参与细胞凋亡、DNA 修复、胞吞作用和细胞周期，并与某些类型的癌症、糖尿病和阿尔茨海默病等疾病状态有关，从而使其成为药物研发的靶点。
蛋白酶体负责蛋白质的回收利用，维持蛋白质合成与降解的平衡。蛋白酶体参与细胞凋亡、DNA 修复、胞吞作用和细胞周期，并与某些类型的癌症、糖尿病和阿尔茨海默病等疾病状态有关，从而使其成为药物研发的靶点。
特点和优势
与高通量处理系统兼容。
适用性
适用于测定培养细胞或细胞裂解物中蛋白酶体复合物相关的糜蛋白酶样蛋白酶活性。
原理
荧光测定蛋白酶体 20S 检测试剂盒是一种均相荧光测定法，可测定培养细胞中与蛋白酶体复合物相关的糜蛋白酶样蛋白酶活性。本试剂盒使用 LLVY-R110 作为蛋白酶体活性的荧光指示剂。蛋白酶体对 LLVY-R110 的裂解产生强绿色荧光 R110，在 520-530 nm下以480-500nm激发波长进行荧光监测。该试验具有耐用性，可适用于高通量测定，以评价蛋白酶体活性或筛选培养细胞或溶液中的抑制剂。该试验可在 96 孔和 384 孔荧光微量滴定板格式下进行。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
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