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Merck
CN

MAK164

Sigma-Aldrich

细胞内过氧化氢检测试剂盒

sufficient for 200 fluorometric tests (green fluorescence)

别名:

细胞中过氧化氢检测

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About This Item

EC 号:
UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 200 fluorometric tests (green fluorescence)

检测方法

fluorometric

相关疾病

aging/geriatric diseases; neurological disorders; pulmonary disorders; orthopedic diseases; cardiovascular diseases

储存温度

−20°C

一般描述

过氧化氢是由分子氧代谢产生的活性氧类物质,既是一种细胞内信号传导信使,也是一种氧化应激来源。过氧化氢由细胞通过多种机制生成,如中性粒细胞和巨噬细胞内NOX介导的ROS产生(呼吸爆发),或者通过在线粒体呼吸期间由电子泄漏产生的超氧阴离子的歧化酶催化产生ROS。异常的过氧化氢产生将导致细胞氧化损伤以及哮喘、动脉粥样硬化、骨质疏松症和神经变性等疾病进展。

应用

细胞内过氧化氢测定试剂盒已被用于测定细胞内的过氧化氢水平。

特点和优势

兼容高通量的操作系统。

适用性

本试剂盒适于检测活体细胞,以及包括细胞抽提物和液体等多种样本的过氧化氢水平。

原理

胞内过氧化氢检测试剂盒提供了一种简便、可重复的定量分析各种样品中(如细胞提取物)过氧化氢水平的方法。该试剂盒采用一种可穿透细胞的传感器,其与过氧化氢反应后产生的荧光产物(λex = 490/λem = 520 nm)可用荧光酶标仪、流式细胞仪和荧光显微镜进行分析。

储存分类代码

10 - Combustible liquids

WGK

WGK 3


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