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用途
sufficient for 200 fluorometric tests
检测方法
fluorometric
相关疾病
endocrinological disorders, diabetes; cancer
储存温度
−20°C
一般描述
在游离半胱氨酸、谷胱甘肽（GSH）和蛋白质半胱氨酸残基中发现的硫醇基团可参与许多生物过程。当两个半胱氨酸残基的硫醇基团被氧化时，所产生的二硫键有助于蛋白质的三级或四级结构。
应用
荧光硫醇测定试剂盒已用于测定样品中以硫醇表示的脂质过氧化。
适用性
该试剂盒适用于检测各种不同生物样品中的硫醇，包括血浆、尿液和细胞提取物。该试剂盒未针对测定蛋白质中的硫醇基团进行优化。
原理
荧光硫醇定量试剂盒可提供超灵敏的测定，可用于定量小分子中的硫醇含量。专有的硫醇感受分子在与含硫醇的化合物反应时可产生强荧光加合物(λex = 490/λem = 520 nm)。此外，硫醇加合物的吸收和发射光谱均与pH无关，使得该测定试剂盒非常稳定。该试剂盒可在100 μL测定体积(10 nM).内检测低至1 皮摩尔的半胱氨酸或GSH。可使用96或384多孔板形式方便地进行测定。
WGK
WGK 3
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Colica C, et al.
Oxidative Medicine and Cellular Longevity, 2017, 14-14 (2017)
Kun Chun et al.
International journal of molecular sciences, 17(9) (2016-09-07)
We investigated the antioxidative activity of LX519290, a derivative of l-allo threonine, in vitro and in vivo. To evaluate the antioxidative activity of LX519290, we performed several in vitro assays (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical-scavenging assays, a ferric
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