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用途
sufficient for 200 fluorometric tests (red)
检测方法
fluorometric
相关疾病
neurological disorders; cancer
储存温度
−20°C
一般描述
活性氧 (ROS) 是由于有氧呼吸过程中氧气减少和细胞内各种酶系统产生的。在生理水平，活性氧有助于细胞信号和宿主防御。ROS 生成增加，高于生物系统的解毒能力，导致氧化应激和细胞损伤。对细胞的主要损伤是由 ROS 诱导的膜脂、必需蛋白质和 DNA 中的多不饱和脂肪酸等大分子的改变所致。ROS 与阿尔茨海默病、帕金森病、癌症、衰老等疾病状态有关。
应用
荧光细胞内活性氧 (ROS) 试剂盒已用于检测活细胞中的细胞内 ROS。
适用性
本试剂盒适于通过酶标仪或荧光显微镜检测细胞内的ROS。
原理
试剂盒提供灵敏的一步荧光测定方法，不用孵育1小时即可检测活细胞的胞内 ROS(特别是超氧化物和羟基自由基)。ROS与细胞透过性传感器反应，生成的荧光产物（λex = 520/λem = 605 nm）与 ROS 量成比例。这一测定可用荧光微板读取仪，在96孔或384孔多孔板中进行。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Neurodegenerative diseases and oxidative stress.
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Nature Reviews. Drug Discovery, 3(3), 205-205 null
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Das K and Roychoudhury A
Frontiers in Endocrinology, 2, 53-53 (2014)
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