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用途
sufficient for 200 fluorometric tests (orange)
应用
cosmetics
food and beverages
检测方法
fluorometric
相关疾病
cancer; neurological disorders
储存温度
−20°C
一般描述
在有氧呼吸期间氧气减少以及在细胞内各种酶体系的作用下，可生成活性氧类物质（ROS）。在生理水平上，ROS有助于细胞信号传导和宿主防御能力。当ROS生成增加至超过生物系统的解毒能力时，将导致氧化应激和细胞损伤。对细胞的主要损伤来源于ROS诱导的大分子改变，如膜脂质的多不饱和脂肪酸、必需蛋白质和DNA。已有研究证明ROS与疾病状态有关，如阿尔茨海默病、帕金森病、癌症和衰老。
应用
细胞内活性氧类物质（ROS）检测试剂盒（荧光法）已可用于评估机械通气（MV）对线粒体的损害。它还被用于测量细胞内ROS的产生。
适用性
本试剂盒适于通过酶标仪、流式细胞仪或带TRITC滤镜的荧光显微镜检测细胞内的ROS。
原理
细胞内ROS检测试剂盒（荧光法）是一种灵敏的一步式荧光测定法，仅需1小时孵育即可检测活细胞中的细胞内ROS（特别是超氧化物和羟基自由基）。ROS与细胞可透过性传感器反应，产生的荧光产物（λex = 540/λem = 570 nm）与ROS含量成比例。该测定可使用方便的96孔或384孔微量滴定板。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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