产品名称
荧光测定细胞内活性氧试剂盒, sufficient for 200 fluorometric tests (orange)
usage
sufficient for 200 fluorometric tests (orange)
application(s)
cosmetics
food and beverages
detection method
fluorometric
relevant disease(s)
cancer; neurological disorders
storage temp.
−20°C
Application
本试剂盒适于通过酶标仪、流式细胞仪或带TRITC滤镜的荧光显微镜检测细胞内的ROS。
细胞内活性氧类物质（ROS）检测试剂盒（荧光法）已可用于评估机械通气（MV）对线粒体的损害。它还被用于测量细胞内ROS的产生。
Biochem/physiol Actions
细胞内ROS检测试剂盒（荧光法）是一种灵敏的一步式荧光测定法，仅需1小时孵育即可检测活细胞中的细胞内ROS（特别是超氧化物和羟基自由基）。ROS与细胞可透过性传感器反应，产生的荧光产物（λex = 540/λem = 570 nm）与ROS含量成比例。该测定可使用方便的96孔或384孔微量滴定板。
General description
在有氧呼吸期间氧气减少以及在细胞内各种酶体系的作用下，可生成活性氧类物质（ROS）。在生理水平上，ROS有助于细胞信号传导和宿主防御能力。当ROS生成增加至超过生物系统的解毒能力时，将导致氧化应激和细胞损伤。对细胞的主要损伤来源于ROS诱导的大分子改变，如膜脂质的多不饱和脂肪酸、必需蛋白质和DNA。已有研究证明ROS与疾病状态有关，如阿尔茨海默病、帕金森病、癌症和衰老。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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