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用途
sufficient for 200 fluorometric tests (Deep red)
应用
cosmetics
food and beverages
检测方法
fluorometric
相关疾病
neurological disorders; cancer
储存温度
−20°C
一般描述
由于在有氧呼吸期间氧气的减少以及细胞内的各种酶系统产生活性氧物质（ROS）。在生理水平，ROS 有助于细胞信号传导和宿主防御。增加的 ROS 产生，高于生物系统的解毒能力，导致氧化应激和细胞损伤。细胞的主要损害是由 ROS 诱导的大分子改变，如膜脂、必需蛋白和 DNA 中的多不饱和脂肪酸。ROS 已经与疾病状态有关，例如阿尔茨海默′病、帕金森′病、癌症和衰老。
应用
- 漂白粉二乙酯在LPS刺激的RAW264.7细胞中通过TLR4/MyD88信号通路显示抗炎和抗氧化活性。：这篇文章利用荧光测定法探讨了漂白粉二乙酯的抗炎和抗氧化特性，证明ROS检测在天然化合物治疗作用分子机制中的作用（Yang S et al., 2019）。
适用性
本试剂盒适于通过酶标仪、流式细胞仪或带TRITC滤镜的荧光显微镜检测细胞内的ROS。
原理
荧光细胞内 ROS 试剂盒提供灵敏的一步荧光检测法，用于在孵育 1 小时后检测活细胞中的细胞内 ROS（特别是超氧化物和羟基自由基）。ROS 与定位于细胞质的荧光传感器反应，产生与存在的 ROS 量成比例的荧光产物。该测定可以 96 或 384 多孔板形式进行，并且在 λex = 650/λem = 675 nm 处进行检测。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
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