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用途
sufficient for 200 tests (UV)
特点
This kit is sufficient for up to 100 assays of 3-hydroxybutyric acid (BOH) and up to 100 assays of acetoacetic acid (AcAc) in 96 well plates.
检测方法
colorimetric
相关疾病
endocrinological disorders, diabetes; neonatal diseases; pediatric diseases; cancer
储存温度
−20°C
一般描述
酮体 3-羟基丁酸 (BOH) 和乙酰乙酸 (AcAc) 主要在肝脏中由脂肪酸氧化生成 ，正常情况下在尿液和血液中浓度较低。血液中酮体浓度升高可能导致代谢性酸中毒，这与糖尿病、儿童期低血糖、生长激素缺乏症、酒精或水杨酸盐中毒以及先天性代谢缺陷相关。
应用
酮体测定法已用于测量人肝癌细胞系 HepG2 培养基中酮体的释放。
特点和优势
兼容高通量的操作系统。
适用性
可用于检测血清、血浆、尿液及其他生物样品中的酮体（乙酰乙酸和3-羟基丁酸）。
原理
在本试剂盒中，使用基于 3-羟基丁酸脱氢酶催化反应的酶分析法测定 AcAc 和 BOH 水平，其中在 340 nm 处测定的 NADH 吸光度更改与 AcAc 和 BOH 浓度直接相关。
储存分类代码
10 - Combustible liquids
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Kim J Shimy et al.
Journal of the Endocrine Society, 4(7), bvaa062-bvaa062 (2020-07-16)
According to the carbohydrate-insulin model of obesity, an elevated insulin-to-glucagon ratio in response to a high-carbohydrate diet directs metabolic fuels toward storage, resulting in lower circulating energy. To determine differences in total circulating energy post-meal related to dietary carbohydrate. Ancillary
Catia Martins et al.
Obesity (Silver Spring, Md.), 28(12), 2331-2338 (2020-11-25)
The purpose of this study was to examine whether the degree of ketosis, measured as plasma β-hydroxybutyrate (βHB) in fasting, was associated with changes in appetite feelings and plasma concentration of appetite-related hormones after weight loss. A total of 87
Role of beta-hydroxybutyric acid in diabetic ketoacidosis: A review.
Stojanovic, et al.
The Canadian Veterinary Journal. La Revue Veterinaire Canadienne, 52(4), 426-426 (2011)
Seshagiri Rao Nandula et al.
Cardiovascular diabetology, 20(1), 44-44 (2021-02-15)
Endothelial progenitor cells (EPCs) has been shown to be dysfunctional in both type 2 diabetes mellitus (T2DM) and chronic kidney disease (CKD) leading to poor regeneration of endothelium and renal perfusion. EPCs have been shown to be a robust cardiovascular
Anila K Madiraju et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(24), E3423-E3430 (2016-06-02)
A key sensor of cellular energy status, AMP-activated protein kinase (AMPK), interacts allosterically with AMP to maintain an active state. When active, AMPK triggers a metabolic switch, decreasing the activity of anabolic pathways and enhancing catabolic processes such as lipid
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