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Merck
CN

MAK122

Sigma-Aldrich

磷脂测定试剂盒

sufficient for 100 colorimetric or fluorometric tests

别名:

Phospholipid Quantification Kit

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

应用

cosmetics
food and beverages
pharmaceutical

检测方法

colorimetric
fluorometric

运输

dry ice

储存温度

−20°C

一般描述

磷脂是一种脂质,是构成细胞膜的重要组成部分,在信号转导中起着重要作用。大多数磷脂包含一分子甘油二酯,一分子磷酸基和一分子胆碱。

在该检测中,磷脂(如卵磷脂、溶血卵磷脂和鞘磷脂)会被酶促水解为胆碱,随后使用胆碱氧化酶和H2O2特异性染料进行测定。反应会产生比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物,此产物与样品中的磷脂浓度成正比。比色法的线性检测范围是3 - 200 μM,荧光法则为0.6 - 20 μM。

应用

磷脂测定试剂盒已用于生化分析 和磷脂定量。

特点和优势

兼容高通量的操作系统。

适用性

适用于生物样品中的磷脂检测。

原理

在该检测中,磷脂(如卵磷脂、溶血卵磷脂和鞘磷脂)会被酶法水解以释放胆碱,而胆碱则可通过胆碱氧化酶和H2O2-特异性染料所测定。所得的比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物与样品中的磷脂浓度成正比。对于比色测定,检测的线性范围是3 - 200 μM;对于荧光测定,检测的线性范围是0.6 - 20 μM。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Aquatic Chronic 3 - Resp. Sens. 1

储存分类代码

10 - Combustible liquids

法规信息

含少量动物源组分生物产品
常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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