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Merck
CN

MAK118

Sigma-Aldrich

转化酶活性检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

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别名:
β-呋喃果糖苷酶检测试剂盒
UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

应用

cosmetics
environmental
food and beverages

检测方法

colorimetric
fluorometric

储存温度

−20°C

一般描述

转化酶(β-呋喃果糖苷酶)是一种催化蔗糖水解为果糖和葡萄糖的酶。转化酶是由某些植物、微生物和蜜蜂产生的。

应用

转化酶检测试剂盒已用于甘蔗转化酶抑制因子重组蛋白的筛选。

特点和优势

兼容高通量的操作系统。

适用性

适用于检测生物及环境样品中的转化酶活性,可用于筛选转化酶抑制因子。

原理

在本试剂盒中,转化酶活性通过偶联酶试验测定,转化酶将蔗糖裂解为葡萄糖和果糖,得到比色 (570 nm)/荧光 (λex= 535 /λem= 587nm) 的产物,与存在的转化酶活性成正比。一个单位的转化酶是在测定条件下在 pH 4.5 下每分钟催化形成 1.0μmole 葡萄糖的酶量。

WGK

WGK 2

法规信息

常规特殊物品

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