用途
sufficient for 100 tests (UV)
检测方法
colorimetric
相关疾病
cancer
储存温度
−20°C
基因信息
human ... GLO1(2739)
mouse ... GLO1(109801)
rat ... GLO1(294320)
一般描述
乙二醛酶I（GLO-1）是一种乳酰基谷胱甘肽裂解酶，也称为甲基乙二醛酶、醛糖脱氧核糖核酸酶、酮醛突变酶和 (R)-S-乳酰基谷胱甘肽甲基乙二醛裂解酶，是一种催化在谷胱甘肽基和醛类之间自发反应中形成的半硫缩醛加合物异构化的酶。乙二醛酶I的主要生理功能是对甲乙二醛的解毒作用、甲乙二醛是一种反应性的2-氧醛、在低浓度下具有抑制细胞生长作用，在毫摩尔浓度下具有细胞毒性。 乙二醛酶I是开发针对细菌、原生动物和癌症的药物的靶向药物。
应用
乙二醛酶I检测试剂盒已被用于评估血浆样品中的乙二醛酶I活性。
特点和优势
兼容高通量的操作系统。
适用性
适用于测定酶制备及生物样品中的乙二醛酶I 活性。可用于检测化合物对乙二醛酶I 活性的影响。
原理
乙二醛酶I活性测定试剂盒提供了一种简单而直接的程序，用于测量多种样品（例如酶制剂或生物样品）中的GLO-1活性。在该测定中，通过监测240nm处的吸光度变化来测量GLO-1介导的S-乳糖基谷胱甘肽的产生。一个单位的乙二醛酶I是在pH 6.6和25℃下每分钟可从甲基乙二醛转化1.0μmol S-乳糖基谷胱甘肽和还原的谷胱甘肽的酶的量。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
Tiago Rodrigues et al.
Pharmacological research, 161, 105198-105198 (2020-09-18)
Methylglyoxal was shown to impair adipose tissue capillarization and insulin sensitivity in obese models. We hypothesized that glyoxalase-1 (GLO-1) activity could be diminished in the adipose tissue of type 2 diabetic obese patients. Moreover, we assessed whether such activity could
Naimesh Solanki et al.
The international journal of neuropsychopharmacology, 20(7), 550-561 (2017-03-25)
Persistent psychological stress often leads to anxiety disorders and depression. Benzodiazepines and selective serotonin reuptake inhibitors are popular treatment options but have limited efficacy, supporting the need for alternative treatment. Based on our recent preclinical work suggesting a causal link
Mandy C Szymanski et al.
Journal of applied physiology (Bethesda, Md. : 1985), 124(2), 330-340 (2017-09-25)
Szymanski MC, Gillum TL, Gould LM, Morin DS, Kuennen MR. Short-term dietary curcumin supplementation reduces gastrointestinal barrier damage and physiological strain responses during exertional heat stress. J Appl Physiol 124: 330-340, 2018. First published September 21, 2017; doi: 10.1152/japplphysiol.00515.2017 .-This
Modulating oxidative stress relieves stress-induced behavioral and cognitive impairments in rats.
Solanki N, et al.
The International Journal of Neuropsychopharmacology, 20(7), 550-561 (2017)
Jin-Quan Huang et al.
Nature chemical biology, 16(3), 250-256 (2020-01-15)
In plants, lineage-specific metabolites can be created by activities derived from the catalytic promiscuity of ancestral proteins, although examples of recruiting detoxification systems to biosynthetic pathways are scarce. The ubiquitous glyoxalase (GLX) system scavenges the cytotoxic methylglyoxal, in which GLXI
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