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用途
sufficient for 100 colorimetric tests
检测方法
colorimetric
相关疾病
cancer; orthopedic diseases; aging/geriatric diseases
储存温度
−20°C
基因信息
human ... PFKL(5211) , PFKM(5213) , PFKP(5214)
mouse ... PFKL(18641) , PFKM(18642) , PFKP(56421)
rat ... PFKL(25741) , PFKM(65152) , PFKP(60416)
一般描述
磷酸果糖激酶 (PFK) 催化糖酵解的第三步，即果糖-6-磷酸转化为果糖-1,6-二磷酸，即糖酵解的限速步骤。PFK 活性除转化后修饰外，还受多种辅助因子的高度调控，可用于测量组织中的糖酵解通量。缺乏 PFK 活性可导致糖原贮积病，糖原贮积病类型 II 型（Tarui 病）——一种糖原贮积病。
应用
磷酸果糖激酶 (PFK) 活性比色试剂盒用于测定磷酸果糖激酶的活性。
适用性
适用于测定磷酸果糖激酶活性，分析葡萄糖代谢和细胞信号，筛选动物组织（肝、脑、心脏、肌肉等）和细胞培养（贴壁细胞或悬浮细胞）中的抗癌药物。
原理
磷酸果糖激酶比色测定试剂盒为测定多种样品中的 PFK 活性提供了一种简单直接的程序。通过偶联酶试验测定 PFK 活性，其中果糖-6-磷酸和 ATP 通过 PFK 转化为果糖-1,6-二磷酸和 ADP。ADP 被酶混合物转化为 AMP 和 NADH。生成的 NADH 还原无色探针，生成与 PFK 活性成比例的比色 (450 nm) 产物。PFK 的 1 个单位是 37°C、pH 7.4 时每分钟生成 1.0 μmol NADH 的酶量。
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