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![2-脱氧-2-[（7-硝基-2,1,3-苯并恶二唑-4-基）氨基]-D-葡萄糖 ≥97% (HPLC)](/deepweb/assets/sigmaaldrich/product/structures/104/527/40bd5a41-ebc4-484e-a10e-891fecfaea79/640/40bd5a41-ebc4-484e-a10e-891fecfaea79.png)
用途
sufficient for 100 colorimetric tests
检测方法
colorimetric
相关疾病
endocrinological disorders, diabetes; cancer
储存温度
−20°C
一般描述
葡萄糖是大多数细胞的主要能量来源。葡萄糖的细胞摄取是葡萄糖代谢的首个限速步骤,该过程受到严密的调控。GLUT家族的转运蛋白可促进葡萄糖摄取，而这些蛋白的表达和活性受到多种机制的调控。许多癌细胞的葡萄糖摄取增加，表现出高速率的有氧糖酵解。胰岛素耐受性细胞在遇到胰岛素刺激时,葡萄糖摄取会减弱。
使用再循环扩增反应比色法（20-100 pmol）,检测限要比荧光法检测（200-1000 pmol,MAK084) 低10倍。
使用再循环扩增反应比色法（20-100 pmol）,检测限要比荧光法检测（200-1000 pmol,MAK084) 低10倍。
应用
葡萄糖摄取检测试剂盒（比色法）已被用于测量各种细胞的葡萄糖摄取。
适用性
适用于检测在96孔微量滴定板中培养的贴壁细胞或悬浮细胞的葡萄糖摄取。
原理
葡萄糖摄取检测试剂盒（比色法）可简单、直接地测定多种细胞的葡萄糖摄取能力。该试剂盒使用葡萄糖类似物2-脱氧葡萄糖（2-DG）测量葡萄糖摄取，2-DG在测定中被细胞摄取并被己糖激酶磷酸化为2-DG6P。2-DG6P无法进入后续代谢步骤，因而会在细胞中聚集，直接成比例地反映细胞的葡萄糖摄取水平。在该测定中,2-DG的摄取水平通过偶联酶促反应方法测定,其中2-DG6P在此过程中被氧化生成NADPH，随后通过再循环扩增反应来测定NAPDH浓度,而后一反应中谷胱甘肽还原酶将利用NADPH生成谷胱甘肽。谷胱甘肽产物与DTNB反应生成TNB，后者可在412 nm波长处检测到。
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Huijuan Shi et al.
Frontiers in oncology, 10, 1034-1034 (2020-08-09)
Colon cancer is one of the most prevalent malignancies that lead to high occurrence of cancer-related deaths. Currently, chemotherapies and radiotherapies remain the primary treatments for advanced colon cancer. Despite the initial effectiveness, a fraction of colon cancer patients developed
Behrouz Etesami et al.
Reports of biochemistry & molecular biology, 9(1), 14-25 (2020-08-22)
Obesity, often associated with insulin resistance and type 2 diabetes, is a metabolic disease that can result in dyslipidemia and hyperglycemia. Many reports describe the hypoglycemic and hypolipidemic properties of the Phoenix dactylifera L. seed extract in STZ-induced diabetic rat
Kyung-Ah Cho et al.
International journal of molecular medicine, 36(3), 839-844 (2015-07-15)
Impaired lipid metabolism and inflammatory pathways have individually been implicated in the development of insulin resistance in skeletal muscle; however, little evidence is available to date linking the two in this context. In this study, we explored a potential molecular
MicroRNA-138 suppresses proliferation, invasion and glycolysis in malignant melanoma cells by targeting HIF-1α.
Chen Y, et al.
Experimental and Therapeutic Medicine, 11(6), 2513-2518 (2016)
PLIN2 inhibits insulin-induced glucose uptake in myoblasts through the activation of the NLRP3 inflammasome.
Cho K A and Peter B K
International Journal of Molecular Medicine, 36(3), 839-844 (2015)
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