usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
General description
葡萄糖是大多数细胞的主要能量来源。葡萄糖的细胞摄取是葡萄糖代谢的首个限速步骤,该过程受到严密的调控。GLUT家族的转运蛋白可促进葡萄糖摄取，而这些蛋白的表达和活性受到多种机制的调控。许多癌细胞的葡萄糖摄取增加，表现出高速率的有氧糖酵解。胰岛素耐受性细胞在遇到胰岛素刺激时,葡萄糖摄取会减弱。
使用再循环扩增反应比色法（20-100 pmol）,检测限要比荧光法检测（200-1000 pmol,MAK084) 低10倍。
使用再循环扩增反应比色法（20-100 pmol）,检测限要比荧光法检测（200-1000 pmol,MAK084) 低10倍。
Application
葡萄糖摄取检测试剂盒（比色法）已被用于测量各种细胞的葡萄糖摄取。
适用于检测在96孔微量滴定板中培养的贴壁细胞或悬浮细胞的葡萄糖摄取。
Biochem/physiol Actions
葡萄糖摄取检测试剂盒（比色法）可简单、直接地测定多种细胞的葡萄糖摄取能力。该试剂盒使用葡萄糖类似物2-脱氧葡萄糖（2-DG）测量葡萄糖摄取，2-DG在测定中被细胞摄取并被己糖激酶磷酸化为2-DG6P。2-DG6P无法进入后续代谢步骤，因而会在细胞中聚集，直接成比例地反映细胞的葡萄糖摄取水平。在该测定中,2-DG的摄取水平通过偶联酶促反应方法测定,其中2-DG6P在此过程中被氧化生成NADPH，随后通过再循环扩增反应来测定NAPDH浓度,而后一反应中谷胱甘肽还原酶将利用NADPH生成谷胱甘肽。谷胱甘肽产物与DTNB反应生成TNB，后者可在412 nm波长处检测到。
Kyung-Ah Cho et al.
International journal of molecular medicine, 36(3), 839-844 (2015-07-15)
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Colon cancer is one of the most prevalent malignancies that lead to high occurrence of cancer-related deaths. Currently, chemotherapies and radiotherapies remain the primary treatments for advanced colon cancer. Despite the initial effectiveness, a fraction of colon cancer patients developed
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Obesity, often associated with insulin resistance and type 2 diabetes, is a metabolic disease that can result in dyslipidemia and hyperglycemia. Many reports describe the hypoglycemic and hypolipidemic properties of the Phoenix dactylifera L. seed extract in STZ-induced diabetic rat
Francisco Javier Ruiz-Ojeda et al.
PloS one, 11(3), e0152550-e0152550 (2016-03-31)
Obesity is characterized by an excessive accumulation of fat in adipose tissue, which is associated with oxidative stress and chronic inflammation. Excessive H2O2 levels are degraded by catalase (CAT), the activity of which is decreased in obesity. We investigated the
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Cho K A and Peter B K
International Journal of Molecular Medicine, 36(3), 839-844 (2015)
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