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Merck
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安全信息

MAK064

Sigma-Aldrich

乳酸检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

别名:

乳酸检测试剂盒

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

应用

cosmetics
food and beverages

检测方法

colorimetric
fluorometric

相关疾病

endocrinological disorders, diabetes; cancer

储存温度

−20°C

一般描述

L(+)-乳酸是一种通过乳酸脱氢酶的作用而在动物中形成的代谢化合物。乳酸是在增殖的细胞中以及在诸如运动的无氧条件下产生的。 异常高浓度的乳酸与癌症、糖尿病和乳酸酸中毒等病理状况有关。L(+)-乳酸是在人体中间代谢中所形成的乳酸的主要立体异构体，并以约1-2 mmol/L的水平存在于血液中。该试剂盒可提供一种简便的方法用于在生物学样本中进行L(+)-乳酸的检测。无需对样本进行预处理或纯化。

应用

乳酸检测试剂盒已用于确定样本中乳酸的浓度。

适用性

适合用于在培养基、发酵培养基、血液和细胞中检测L(+)-乳酸。

原理

在该测定中，乳酸的浓度是通过一种酶检测而被测定的，其可产生与存在的乳酸成比例的比色（570nm）/荧光测定（λex = 535 nm/λem = 587 nm）产物。该试剂盒的常见灵敏度水平在0.001-10 mM之间。

替代产品

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317,H334

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

10 - Combustible liquids

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can I make up my own Lactate standard to use with Product MAK064, Lactate Assay kit?

    Unfortunately, no. If you run the 100 mM L-(+)-Lactate standard (MAK064D) that comes with the kit, you should get good results. However, if you just use, for example, a solution of sodium L-(+)-lactate dissolved in water or buffer, you probably won't get good results. There are cofactors present in biological samples and in the L-(+)-Lactate standard that comes with the kit (which is not a pure solution of sodium L-(+)-Lactate in water or buffer). If such a "homemade" standard solution were to be spiked with a biological sample or the kit standard, the calculations would be more complicated, but the assay should work.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Min Xu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 47(1), 151-160 (2018-05-16)
Poor response to chemotherapy leads to the relapse and metastatic progression of tumors. Reprogrammed glucose metabolism is one of the important hallmarks of cancer that facilitates cancer cell survival, proliferation and chemoresistance. However, the precise fate of glucose metabolism and
Bibi Uhre Nielsen et al.
PloS one, 15(9), e0238524-e0238524 (2020-09-12)
Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs)
Prabha Shrestha et al.
PLoS pathogens, 13(9), e1006628-e1006628 (2017-09-19)
Primary effusion lymphoma (PEL) is an aggressive B-cell lymphoma with poor prognosis caused by Kaposi's sarcoma-associated herpesvirus (KSHV). Previous studies have revealed that HIF-1α, which mediates much of the cellular response to hypoxia, plays an important role in life cycle
Annasofia Anemone et al.
International journal of oncology, 51(2), 498-506 (2017-07-18)
Dichloroacetate (DCA) can reverse the glycolytic phenotype that is responsible of increased lactate production and extracellular pH acidification in cancer cells. Magnetic resonance imaging-chemical exchange saturation transfer (MRI-CEST) pH mapping is a novel non-invasive imaging approach that can measure in vivo
Cardiac STAT3 Deficiency Impairs Contractility and Metabolic Homeostasis in Hypertension.
Altara R, et al.
Frontiers in Pharmacology, 7, 436-436 (2016)
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