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用途
sufficient for 100 colorimetric or fluorometric tests
应用
cosmetics
food and beverages
检测方法
colorimetric
fluorometric
相关疾病
cancer
储存温度
−20°C
一般描述
柠檬酸是三羧酸（TCA）循环的关键中间体，通过向乙酰辅酶A的乙酰基中加入草酰乙酸而得。柠檬酸通过柠檬酸-苹果酸梭子从线粒体中转运出来，然后转化回乙酰辅酶A进行脂肪酸合成。柠檬酸是脂肪酸合成和糖酵解的别构调节剂，分别通过作用于乙酰辅酶A羧化酶和磷酸果糖激酶来实现。由于性别、年龄和各种其他因素（包括疾病状态），柠檬酸的代谢和分布差异可能很大。前列腺癌细胞中的细胞柠檬酸水平降低，柠檬酸水平可能是前列腺癌生长速率的标志物。柠檬酸检测试剂盒提供了一种可简单、灵敏、快速地对各种样品中的柠檬酸进行定量的方法。
应用
柠檬酸检测试剂盒用于检测样品中柠檬酸的浓度。
适用性
适用于对包括组织和细胞在内的多种样品中的柠檬酸盐进行测定
原理
柠檬酸的浓度通过酶偶联法检测，产生与存在的柠檬酸成比例的比色（570 nm）/荧光（λex = 535/λem = 587 nm）产物。柠檬酸检测试剂盒的灵敏度在各种样品中通常为0.1至10 nM（2 μmM-10 mM）柠檬酸。
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