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用途
sufficient for 100 colorimetric or fluorometric tests
应用
cosmetics
food and beverages
检测方法
colorimetric
fluorometric
相关疾病
cancer
储存温度
−20°C
一般描述
柠檬酸是三羧酸（TCA）循环的关键中间体，通过向乙酰辅酶A的乙酰基中加入草酰乙酸而得。柠檬酸通过柠檬酸-苹果酸梭子从线粒体中转运出来，然后转化回乙酰辅酶A进行脂肪酸合成。柠檬酸是脂肪酸合成和糖酵解的别构调节剂，分别通过作用于乙酰辅酶A羧化酶和磷酸果糖激酶来实现。由于性别、年龄和各种其他因素（包括疾病状态），柠檬酸的代谢和分布差异可能很大。前列腺癌细胞中的细胞柠檬酸水平降低，柠檬酸水平可能是前列腺癌生长速率的标志物。柠檬酸检测试剂盒提供了一种可简单、灵敏、快速地对各种样品中的柠檬酸进行定量的方法。
应用
柠檬酸检测试剂盒用于检测样品中柠檬酸的浓度。
适用性
适用于对包括组织和细胞在内的多种样品中的柠檬酸盐进行测定
原理
柠檬酸的浓度通过酶偶联法检测，产生与存在的柠檬酸成比例的比色（570 nm）/荧光（λex = 535/λem = 587 nm）产物。柠檬酸检测试剂盒的灵敏度在各种样品中通常为0.1至10 nM（2 μmM-10 mM）柠檬酸。
相关产品
危险声明
预防措施声明
危险分类
Aquatic Chronic 3
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
188.6 °F - closed cup
闪点(°C)
87 °C - closed cup
法规信息
监管及禁止进口产品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
High-intensity sprint training inhibits mitochondrial respiration through aconitase inactivation.
Larsen F J, et al.
Faseb Journal, 30(1), 417-427 (2015)
Long Ding et al.
The EMBO journal, 37(17) (2018-07-28)
Seipin, the gene that causes Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2), is important for adipocyte differentiation and lipid homeostasis. Previous studies in Drosophila revealed that Seipin promotes ER calcium homeostasis through the Ca2+-ATPase SERCA, but little is known about the
Cheng Zhang et al.
Cell reports, 21(8), 2058-2065 (2017-11-23)
We discovered that induced pluripotent stem cell (iPSC) clones generated from aged tissue donors (A-iPSCs) fail to suppress oxidative phosphorylation. Compared to embryonic stem cells (ESCs) and iPSCs generated from young donors (Y-iPSCs), A-iPSCs show poor expression of the pluripotent
Thi Mong Diep Nguyen et al.
PloS one, 10(7), e0134420-e0134420 (2015-07-30)
Semen cryopreservation is a unique tool for the management of animal genetic diversity. However, the freeze-thaw process causes biochemical and physical alterations which make difficult the restoration of sperm energy-dependent functions needed for fertilization. 5'-AMP activated protein kinase (AMPK) is
Jade D Bailey et al.
Cell reports, 28(1), 218-230 (2019-07-04)
Classical activation of macrophages (M(LPS+IFNγ)) elicits the expression of inducible nitric oxide synthase (iNOS), generating large amounts of NO and inhibiting mitochondrial respiration. Upregulation of glycolysis and a disrupted tricarboxylic acid (TCA) cycle underpin this switch to a pro-inflammatory phenotype.
商品
Fatty acid synthesis supports cancer cell proliferation, essential for membrane generation, protein modification, and bioenergetics.
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