用途
sufficient for 100 colorimetric or fluorometric tests
检测方法
colorimetric
fluorometric
相关疾病
gastrointestinal diseases
运输
wet ice
储存温度
−20°C
基因信息
human ... GPT(2875)
mouse ... GPT(76282)
rat ... GPT(81670)
一般描述
丙氨酸氨基转移酶(ALT),也称为血清谷氨酸-丙酮酸转氨酶(SGPT),是一种吡哆醛-磷酸盐依赖型的酶,其可催化氨基从丙氨酸到α-酮戊二酸的可逆型转移,并生成丙酮酸和谷氨酸。ALT主要在肝脏和血清中被发现,但也存在于其他组织中。肝细胞损伤经常会导致血清ALT水平的升高而血清ALT水平可被用作肝损伤的一种标志物。
应用
ALT活性检测试剂盒已用于确定血清样品中丙氨酸氨基转移酶(ALT)的活性。
特点和优势
兼容高通量处理系统。
适用性
适于测定组织、细胞和血清等生物学样品中的丙氨酸转氨酶(ALT)活性。
原理
ALT活性检测试剂盒可提供在多种生物学样本进行简单而直接的ALT活性测定过程。
ALT活性由一种偶联酶检测所确定,其可产生与生成的丙酮酸盐成比例的比色(570 nm)/荧光(λex = 535/λem = 587 nm)产物。一个单位的ALT定义为在 37 °C每分钟可生成1.0 μ摩尔丙酮酸所需的酶量。
ALT活性由一种偶联酶检测所确定,其可产生与生成的丙酮酸盐成比例的比色(570 nm)/荧光(λex = 535/λem = 587 nm)产物。一个单位的ALT定义为在 37 °C每分钟可生成1.0 μ摩尔丙酮酸所需的酶量。
警示用语:
Danger
危险声明
危险分类
Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 3
法规信息
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