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Merck
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安全信息

MAK045

Sigma-Aldrich

HDL 和 LDL/VLDL 定量试剂盒

sufficient for 100 colorimetric or fluorometric tests

别名:

HDL和LDL/VLDL检测试剂盒

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.25

用途

sufficient for 100 colorimetric or fluorometric tests

应用

cosmetics
food and beverages

检测方法

colorimetric
fluorometric

相关疾病

cardiovascular diseases

储存温度

−20°C

一般描述

脂蛋白转运大部分血浆脂质，包括胆固醇和甘油三酯。高密度脂蛋白（HDL）、低密度脂蛋白（LDL）和极低密度脂蛋白（VLDL）是导致血液中绝大多数胆固醇转运的脂蛋白。高 LDL 水平和低 HDL 水平与不良心血管事件风险增加密切相关。

应用

HDL 和 LDL/VLDL 定量试剂盒已用于测定血清样品中 HDL 和 LDL/VLDL 的浓度。

适用性

适用于血浆和血清样本。

原理

在该试剂盒中，首先分离血清 HDL 和 LDL/VLDL，然后通过偶联酶测定法测定各自的胆固醇浓度，得到与存在的胆固醇成比例的比色（570nm）/荧光（λex = 535/λem = 587 nm）产物。该试剂盒也可用于测定样品中存在的游离胆固醇和胆固醇酯的浓度。

相关产品

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

10 - Combustible liquids

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can these kits be used with serum samples from mouse?

    Yes, this kit can be used on mouse serum samples. We generally recommend that fresh serum samples be used. Frozen samples can probably be used. However, the successful use of frozen samples depends on the efficiency of freezing them and the amount of time they have been frozen. If previously frozen serum samples are used, you may want to check the performance of the kit with fresh vs. frozen samples.

  6. For Product MAK045, HDL and LDL/VLDL Quantitation Kit, instead of using microcentrifuge tubes in the sample preparation, have you developed a method that uses a 96-well filter plate?

    For product MAK045, HDL and LDL/VLDL Quantitation Kit, we have not used a filter plate. The common problem with using filters is that lipids tend to bind to or get stuck in them. Using a filter plate would probably give decent results for the HDL solution that would go through the filter. However, it would be very difficult to get the LDL/VLDL precipitate resuspended completely if it is on a filter membrane in a 96-well plate.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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