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用途
sufficient for 100 colorimetric tests
检测方法
colorimetric
相关疾病
cancer
储存温度
−20°C
一般描述
烟酰胺腺嘌呤二核苷酸(NAD)是一种参与多种氧化还原反应的酶辅因子。NAD作为电子载体，在氧化(NAD)和还原(NADH)形式之间循环。NAD除了在氧化还原反应中发挥作用外，在ADP(二磷酸腺苷)-核糖基化反应和作为去乙酰化酶的底物中也发挥着关键作用。
应用
NAD/NADH定量试剂盒已可用于，当存在曲美他嗪时，SIRT1(去乙酰化酶1)活性(与缺血再灌注损伤相关)对NAD+/NADH定量的影响。
适用性
适用于动物组织(肝、肾等)、细胞培养(贴壁细胞或悬浮细胞)、血清或尿液中NADH和NAD的检测。
原理
NAD/NADH定量试剂盒为敏感检测细胞内核苷酸、NAD(烟酰胺腺嘌呤二核苷酸)和NADH及它们的比值提供了一种方便的工具，无需从样品中纯化NAD/NADH。该试剂盒针对NAD和NADH，不检测NADP或NADPH。NADt(NAD和NADH)或NADH在比色法(450 nm)中定量。
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警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
10 - Combustible liquids
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Mammalian sirtuins?emerging roles in physiology, aging, and calorie restriction.
Haigis M C and Leonard P G
Genes & Development, 20(21), 2913-2921 (2006)
Frédérique Moyrand et al.
Eukaryotic cell, 7(12), 2069-2077 (2008-09-30)
The genome of the basidiomycete pathogenic yeast Cryptococcus neoformans carries two UDP-glucose epimerase genes (UGE1 and UGE2). UGE2 maps within a galactose cluster composed of a galactokinase homologue gene and a galactose-1-phosphate uridylyltransferase. This clustered organization of the GAL genes
Deepanwita Banerjee et al.
BMC systems biology, 11(1), 51-51 (2017-04-28)
The leading edge of the global problem of antibiotic resistance necessitates novel therapeutic strategies. This study develops a novel systems biology driven approach for killing antibiotic resistant pathogens using benign metabolites. Controlled laboratory evolutions established chloramphenicol and streptomycin resistant pathogens
Andrea M Lencina et al.
mBio, 9(4) (2018-07-05)
The opportunistic pathogen Streptococcus agalactiae is the major cause of meningitis and sepsis in a newborn's first week, as well as a considerable cause of pneumonia, urinary tract infections, and sepsis in immunocompromised adults. This pathogen respires aerobically if heme
Haiping Wang et al.
Nature immunology, 21(3), 298-308 (2020-02-19)
Depleting regulatory T cells (Treg cells) to counteract immunosuppressive features of the tumor microenvironment (TME) is an attractive strategy for cancer treatment; however, autoimmunity due to systemic impairment of their suppressive function limits its therapeutic potential. Elucidating approaches that specifically
商品
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Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.
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Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.
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