用途
sufficient for 100 fluorometric tests
检测方法
fluorometric
相关疾病
cancer
储存温度
−20°C
一般描述
果糖-6-磷酸（F6P）是一种通过磷酸葡萄糖异构酶异构化葡萄糖-6-磷酸产生的糖酵解途径中间体。F6P可被进一步磷酸化为果糖1,6-二磷酸，随后可裂解为磷酸甘油醛和磷酸二羟丙酮。F6P也可以从糖酵解分流至磷酸戊糖途径的非氧化分支，以生成用于核苷酸合成的戊糖磷酸。F6P水平在快速增殖的细胞（如癌细胞）中升高。果糖-6-磷酸测定试剂盒是一种高灵敏度和简单的基于荧光的方法，可用于对多种样品中的F6P进行定量。
应用
果糖-6-磷酸测定试剂盒已被用于测定果糖-6-磷酸（F-6-P） 和葡萄糖-6-磷酸（G6P）。
适用性
适用于对多种样品中的果糖-6-磷酸进行定量
原理
果糖-6-磷酸的浓度可通过偶联酶反应进行测定，该反应可产生荧光测定产物（λex = 535nm /λem = 587nm），其与存在的果糖-6-磷酸成比例。该试剂盒的一般灵敏度为0.01至0.5纳摩尔F6P。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
10 - Combustible liquids
闪点(°F)
188.6 °F - closed cup
闪点(°C)
87 °C - closed cup
法规信息
常规特殊物品
Minle Li et al.
Cell death & disease, 13(2), 99-99 (2022-02-04)
Transketolase (TKT) which is an important metabolic enzyme in the pentose phosphate pathway (PPP) participates in maintaining ribose 5-phosphate levels. TKT is necessary for maintaining cell growth. However, we found that in addition to this, TKT can also affect tumor
Reciprocal regulation between O-GlcNAcylation and tribbles pseudokinase 2 (TRIB2) maintains transformative phenotypes in liver cancer cells.
Yao B, et al.
Cellular Signalling, 28(11) (2016)
Kenichi Matsumoto et al.
Cancer letters, 500, 29-40 (2020-12-12)
Glycolysis emerges as a new therapeutic target for malignancies. The inhibition of glycolytic activator, PFKFB3, repairs tumor endothelial cell function, and normalizing the tumor microenvironment. We aimed to investigate the significance of PFKFB3 in HCC, and the effects of the
Sicheng Gao et al.
DNA and cell biology, 38(4), 286-296 (2019-02-15)
Abnormal expression of O-Linked β-N-acetylglucosamine (O-GlcNAc) and β-catenin is a general feature of cancer and contributes to transformed phenotypes. In this study, we identified the interaction between O-GlcNAc and β-catenin, and explored their effects on the progression of liver cancer.
Lauriane Galle-Treger et al.
The Journal of allergy and clinical immunology, 145(2), 502-517 (2019-11-19)
Allergic asthma is a chronic inflammatory disorder characterized by airway hyperreactivity (AHR) and driven by TH2 cytokine production. Group 2 innate lymphoid cells (ILC2s) secrete high amounts of TH2 cytokines and contribute to the development of AHR. Autophagy is a
商品
本页面介绍了一篇有关瓦博格效应的文章，以及其如何能够在正常氧气水平下，增强肿瘤细胞中葡萄糖向乳酸的转化。Otto Heinrich Warburg在1924年证明，癌细胞显示出对糖酵解的依赖性增加，以满足他们的能量需求，无论是否有充足的氧气存在。
Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门