usage
sufficient for 100 colorimetric or fluorometric tests
application(s)
cosmetics
food and beverages
detection method
colorimetric, fluorometric
relevant disease(s)
endocrinological disorders, diabetes
storage temp.
−20°C
General description
蔗糖是一种二糖,蔗糖酶水解葡萄糖和果糖。葡萄糖是细胞呼吸中用于能量产生的主要碳水化合物。当细胞摄取时,葡萄糖能够被糖酵解分解或聚合用于能量储存。
Application
葡萄糖和蔗糖检测试剂盒已被用于:
- 测定蔗糖、有氧糖酵解和丙酮酸激酶活性
- 测定草莓中的蛋白质和糖
- 测定小鼠血浆胰岛素和葡萄糖水平
- 分析血浆葡萄糖和血浆乳酸并定量测定葡萄糖/蔗糖
适合于各种生物样本,包括血清,血浆,其他体液,食物和生长因子。
Biochem/physiol Actions
在该检测试剂盒中,葡萄糖被葡萄糖氧化酶氧化,生成的比色(570nm)/荧光 (λex = 535 nm/λem = 587 nm) 产物和葡萄糖含量成正比。为了测量蔗糖,会在反应中加入蔗糖酶,将蔗糖转化成葡萄糖和果糖。总葡萄糖减去自由葡萄糖就是蔗糖的浓度。该试剂盒检测线性范围,葡萄糖荧光分析是0.2-1.0nmole,葡萄糖比色分析是2-10nmole。
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1
存储类别
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
法规信息
常规特殊物品
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