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Merck
CN

MAK013

Sigma-Aldrich

葡萄糖和蔗糖检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

别名:

葡萄糖和蔗糖比色/荧光分析试剂盒

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

应用

cosmetics
food and beverages

检测方法

colorimetric
fluorometric

相关疾病

endocrinological disorders, diabetes

储存温度

−20°C

一般描述

蔗糖是一种二糖,蔗糖酶水解葡萄糖和果糖。葡萄糖是细胞呼吸中用于能量产生的主要碳水化合物。当细胞摄取时,葡萄糖能够被糖酵解分解或聚合用于能量储存。

应用

葡萄糖和蔗糖检测试剂盒已被用于:
  • 测定蔗糖、有氧糖酵解和丙酮酸激酶活性
  • 测定草莓中的蛋白质和糖
  • 测定小鼠血浆胰岛素和葡萄糖水平
  • 分析血浆葡萄糖和血浆乳酸并定量测定葡萄糖/蔗糖

适用性

适合于各种生物样本,包括血清,血浆,其他体液,食物和生长因子。

原理

在该检测试剂盒中,葡萄糖被葡萄糖氧化酶氧化,生成的比色(570nm)/荧光 (λex = 535 nm/λem = 587 nm) 产物和葡萄糖含量成正比。为了测量蔗糖,会在反应中加入蔗糖酶,将蔗糖转化成葡萄糖和果糖。总葡萄糖减去自由葡萄糖就是蔗糖的浓度。该试剂盒检测线性范围,葡萄糖荧光分析是0.2-1.0nmole,葡萄糖比色分析是2-10nmole。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Aquatic Chronic 3 - Resp. Sens. 1

储存分类代码

10 - Combustible liquids

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup

法规信息

常规特殊物品

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分析证书(COA)

Lot/Batch Number

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访问文档库

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Nuno F Silva-Soares et al.
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