推荐产品
用途
sufficient for 100 fluorometric tests
检测方法
fluorometric
相关疾病
neonatal diseases; neurological disorders; pediatric diseases
储存温度
−20°C
一般描述
苯丙氨酸是一种非极性必需氨基酸。在肝脏中，苯丙氨酸转化为酪氨酸，酪氨酸是包括 L -DOPA 和黑色素在内的多种化合物的前体。苯丙氨酸羟化酶活性缺陷导致遗传代谢病苯丙酮尿症 (PKU)。苯丙酮尿症导致苯丙氨酸和苯丙氨酸代谢产物的积累，如果不治疗，可导致生长缺陷和智力发育迟缓。
适用性
适用于细胞和组织培养上清、血清及其他生物样本中 L-苯丙氨酸检测
原理
通过偶联酶试验测定苯丙氨酸浓度，通过该试验可生产苯丙氨酸脱氨基和 NADH，NADH 与探针反应生成与存在的苯丙氨酸成比例的荧光 (λex = 535 nm/λem = 587 nm) 产物。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
No data available
闪点(°C)
No data available
法规信息
常规特殊物品
Phenylketonuria: an inborn error of phenylalanine metabolism.
Williams R A, et al.
The Clinical Biochemist. Reviews / Australian Association of Clinical Biochemists, 29(1), 31-31 (2008)
AMINO ACIDS | Metabolism
Encyclopedia of Food Sciences and Nutrition (Second Edition) (2003)
Nathan Crook et al.
ACS synthetic biology, 9(5), 1010-1021 (2020-04-24)
The development of robust engineered probiotic therapies demands accurate knowledge of genetic construct expression in the gut. However, the monetary and ethical costs of testing engineered strains in vertebrate hosts are incompatible with current high-throughput design-build-test cycles. To enable parallel
CTI Reviews
Clinical Chemistry, Principles, Procedures, Correlations (2016)
Nathan Crook et al.
Cell host & microbe, 25(4), 499-512 (2019-03-31)
Probiotics are living microorganisms that are increasingly used as gastrointestinal therapeutics by virtue of their innate or engineered genetic function. Unlike abiotic therapeutics, probiotics can replicate in their intended site, subjecting their genomes and therapeutic properties to natural selection. We exposed
实验方案
Assay protocol for the fluorometric detection of Phenylalanine in biological samples using the phenylalanine assay kit.
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