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M9943

Sigma-Aldrich

10X MTP Taq Buffer

10X buffer, free of DNA contaminants; use with MTP Taq DNA Polymerase

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About This Item

UNSPSC代码:
41106306
NACRES:
NA.52

一般描述

DNA contaminants can be introduced into PCR through a number of reagents. To minimize the risk of contaminant DNA during PCR, we offer 10x MTP Taq buffer to be used with MTP Taq DNA Polymerase (Product No. D7442). Each lot of MTP Taq buffer undergoes strict quality control testing to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.

应用

10X MTPTaq Buffer has been used:

  • for the amplification of bacterial 16s rRNA genes from purified DNA
  • to amplify archaeal 16s rRNA
  • along with MTP Taq DNA polymerase (D7442) to amplify specific bacterial 16s rRNA genes from purified DNA

法律信息

MTP is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Camille Mazière et al.
Microbiological research, 252, 126854-126854 (2021-08-29)
In order to understand the effect of human practices on microbial mats organisation, the study aimed to investigate the biodiversity within microbial mats from exploited and abandoned salterns. Despite several attempts, archaeal 16S rRNA gene fragment sequences were not obtained
Florie Maillard et al.
PloS one, 14(4), e0214660-e0214660 (2019-04-10)
Increased visceral adipose tissue and dysbiosis in the overweight and obese promote chronic inflammation. The aim of this study was to compare the effects of moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT) on the gut-adipose tissue cross-talk in
Florie Maillard et al.
Cells, 8(1) (2019-01-13)
Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics
Florie Maillard et al.
Cells, 8(1) (2019-01-13)
Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics
C E Corless et al.
Journal of clinical microbiology, 38(5), 1747-1752 (2000-05-02)
A set of universal oligonucleotide primers specific for the conserved regions of the eubacterial 16S rRNA gene was designed for use with the real-time PCR Applied Biosystems 7700 (TaqMan) system. During the development of this PCR, problems were noted with

实验方案

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.

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