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Merck
CN

M9309

Sigma-Aldrich

McCoy 5A培养基

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

别名:

5A basal medium

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About This Item

MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.71

产品名称

McCoy 5A培养基, Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

质量水平

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)

运输

ambient

储存温度

2-8°C

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一般描述

McCoy 及其同事报道了体外培养 Novikoff 肝癌细胞所需的氨基酸。这些实验使用的是基础培养基 5A,该培养基后来被改良产生 McCoy′s 5A 培养基。

应用

McCoy′s 5A 培养基已用于:
  • 培养 HCT-116?细胞
  • 培养透明细胞肾细胞癌(ccRCC)细胞 Caki-1
  • 维持和培养人骨肉瘤 Saos-2 细胞系,用于体外细胞研究

生化/生理作用

McCoys 5A培养基起初是通过修改BME中的氨基酸,用来支持肝肿瘤细胞。 该配方还被用来支持骨髓、皮肤、牙龈、肾脏、大网膜、肾上腺、肺、脾、大鼠胚胎和其他细胞类型原代培养的生长。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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