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质量水平
无菌性
sterile-filtered
形式
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)
运输
ambient
储存温度
2-8°C
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一般描述
McCoy 及其同事报道了体外培养 Novikoff 肝癌细胞所需的氨基酸。这些实验使用的是基础培养基 5A，该培养基后来被改良产生 McCoy′s 5A 培养基。
应用
McCoy′s 5A 培养基已用于：
- 培养 HCT-116？细胞
- 培养透明细胞肾细胞癌（ccRCC）细胞 Caki-1
- 维持和培养人骨肉瘤 Saos-2 细胞系，用于体外细胞研究
生化/生理作用
McCoys 5A培养基起初是通过修改BME中的氨基酸，用来支持肝肿瘤细胞。 该配方还被用来支持骨髓、皮肤、牙龈、肾脏、大网膜、肾上腺、肺、脾、大鼠胚胎和其他细胞类型原代培养的生长。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Aleksandr Ianevski et al.
Cancers, 12(6) (2020-07-08)
The evidence that pan-Bcl-2 or Bcl-xL-specific inhibitors prematurely kill virus-infected or RNA/DNA-transfected cells provides rationale for investigating these apoptotic inducers further. We hypothesized that not only invasive RNA or DNA (biological factors) but also DNA/RNA-damaging chemical or physical factors could
Nengquan Sheng et al.
Cancer medicine, 6(6), 1331-1340 (2017-04-26)
MicroRNA-145 (miR-145), as a tumor-suppressive miRNA, has been demonstrated down-regulated in colorectal cancer (CRC) cells, and could inhibit CRC cells growth. However, the molecular pathway in which miR-145 modulates CRC malignant transformation has not been fully revealed. Here, we reported
Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells.
Xiang-Ping Liu et al.
Journal of experimental & clinical cancer research : CR, 28, 52-52 (2009-04-21)
RhoA and RhoC are deregulated by over expression in many human tumors, including colorectal cancer. Some reports show that they play a pivotal role in the carcinogenesis, tumor development and infiltration metastasis. In this study, for the first time we
Naomi D Willis et al.
PloS one, 3(8), e2988-e2988 (2008-08-21)
A-type lamins are type V intermediate filament proteins encoded by the gene LMNA. Mutations in LMNA give rise to diverse degenerative diseases related to premature ageing. A-type lamins also influence the activity of the Retinoblastoma protein (pRb) and oncogenes such
Qiao Zhang et al.
International journal of oncology, 57(1), 197-212 (2020-04-23)
Glucose‑6‑phosphate dehydrogenase (G6PD) is crucial rate‑limiting enzyme of the pentose phosphate pathway (PPP). G6PD dysregulation has been reported in various types of human cancer, and the role of G6PD in cancer progression was demonstrated in numerous studies. A previous study
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