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一般描述
牛心脏的苹果酸脱氢酶在 NAD 结合活性位点包含一个组氨酸残基，该残基对活性至关重要。当该组氨酸突变时，观察到活性丧失。
苹果酸脱氢酶是一种细胞质同工酶，是三羧酸循环中的重要催化剂。
应用
苹果酸脱氢酶已用于评估流动注射系统的研究，用于在线监测生物过程中的富马酸。 它也曾被用于一项研究，调查一种寄生在德州花生上的根结线虫。
单位定义
在pH7.5,25℃条件下，一个单位每分钟可将1.0 μmol的草酰乙酸和β-NADH转化为 L苹果酸和β-NAD。
外形
3 M (NH4)2SO4 - 0.01 M KH2PO4 溶液 (pH 7.3) 中的混悬液
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Eisaku Hokazono et al.
Clinica chimica acta; international journal of clinical chemistry, 412(15-16), 1436-1440 (2011-05-10)
In human serum, as for phospholipids not containing choline, phosphatidylethanolamine (PE) exists approximately 5% in a whole phospholipid. PE is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis
Flow injection system for on-line monitoring of fumaric acid in biological processes
Rhee, J.
Analytica Chimica Acta, 499, 71-80 (2003)
Z Y Chen et al.
Plant physiology, 112(2), 677-684 (1996-10-01)
At low-CO2 (air) conditions, the unicellular green alga Chlamydomonas reinhardtii acquires the ability to raise its internal inorganic carbon concentration. To study this adaptation to low CO2, cDNA clones induced under low-CO2 growth conditions were selected through differential screening. One
J D Eisenback et al.
Journal of nematology, 35(4), 395-403 (2003-12-01)
Meloidogyne haplanaria n. sp. is described and illustrated from specimens parasitizing peanut in Texas. The perineal pattern of the female is rounded to oval with a dorsal arch that is high and rounded except for striae near the vulva, which
Vladimir B Ritov et al.
Analytical biochemistry, 333(1), 27-38 (2004-09-08)
This study addresses an application of pyridine nucleotide enzymatic analyses to evaluate the activity of the mitochondrial electron transport chain (reduced nicotinamide adenine dinucleotide (NADH) oxidase) and Complexes I and II in samples of human muscle as small as approximately
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
实验方案
Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.
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