推荐产品
应用
矿物油已用作微滴胚胎培养中的覆盖层。
注意
该产品并不无菌。矿物油难以灭菌。它只能通过仔细过滤温热的油来灭菌。假定抗生素将会被加入到细胞培养基中。否则，应使用货号M5310，因为它是经无菌过滤的。
分析说明
该材料在微滴胚胎培养物中作为覆盖物已经过测试。（所有经检测的胚胎材料都是使用小鼠胚胎进行检测的。）通常使用BCF1 x BDF1杂合体。该油主要用于防止培养基蒸发，特别是在进行胚胎操作时。1相应的步骤如下：单细胞（BCF1 X BDF1）胚胎在三重复的补充含有0.4% BSA的经胚胎检测的HTF（人输卵管液）培养基微滴中进行培养。如需验证检测，至少70%的单细胞阶段对照胚胎必须在96小时发育至胚泡期。如需产品发布，至少80%的单细胞阶段检测胚胎利用待测材料必须在96小时发育至胚泡期。
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
No data available
闪点(°C)
No data available
个人防护装备
Eyeshields, Gloves
Minoru Kubo et al.
Nucleic acids research, 47(9), 4539-4553 (2019-03-16)
Next-generation sequencing technologies have made it possible to carry out transcriptome analysis at the single-cell level. Single-cell RNA-sequencing (scRNA-seq) data provide insights into cellular dynamics, including intercellular heterogeneity as well as inter- and intra-cellular fluctuations in gene expression that cannot
Production of a gonadotropin-releasing hormone 2 receptor knockdown (GNRHR2 KD) swine line
Desaulniers AT, et al.
Transgenic research, 26(4), 567-575 (2017)
TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing
Bagheri H, et al.
The CRISPR journal, 1(3), 223-229 (2018)
Insulin and branched-chain amino acid depletion during mouse preimplantation embryo culture programmes body weight gain and raised blood pressure during early postnatal life
Velazquez M
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(2), 590-600 (2018)
Mariana Schroeder et al.
Nature communications, 9(1), 1596-1596 (2018-04-25)
Anorexia nervosa (AN) is a devastating eating disorder characterized by self-starvation that mainly affects women. Its etiology is unknown, which impedes successful treatment options leading to a limited chance of full recovery. Here, we show that gestation is a vulnerable
商品
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
实验方案
使用CRISPR/Cas9系统生成正确的转基因小鼠需要充分理解正在测试的假设。了解如何创建成功的小鼠模型项目。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门