推荐产品
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
9B10, monoclonal
表单
buffered aqueous solution
分子量
antigen ~85 kDa
种属反应性
human
浓度
~2 mg/mL
技术
immunocytochemistry: 10-20 μg/mL using HeLa cells
immunoprecipitation (IP): suitable
microarray: suitable
同位素/亚型
IgG2b
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MAD1L1(8379)
一般描述
Mitotic arrest deficient-like 1 (MAD1) is part of the cell-cycle checkpoint.
Monoclonal Anti-Mad1 (mouse IgG2b isotype) is derived from the hybridoma 9B10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with human recombinant Mad1 protein.
免疫原
recombinant human Mad1 protein.
应用
Monoclonal Anti-Mad1 antibody produced in mouse has been used in
- immunoblotting
- immunoprecipitation
- immunocytochemistry
生化/生理作用
Mitotic arrest deficient-like 1 (MAD1) functions as a regulator in the segregation of chromosomes during mitosis. It recruits mitotic arrest deficient-like 2 (MAD2) to kinetochores and directs it to function as an inhibitor. MAD1 also helps MAD2 to associate with translocated promoter region (TPr).
外形
Solution in 0.01 M phosphae buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Jun Wan et al.
Current biology : CB, 24(22), 2687-2692 (2014-12-03)
Mitotic arrest deficient 1 (Mad1) plays a well-characterized role in the major cell-cycle checkpoint that regulates chromosome segregation during mitosis, the mitotic checkpoint (also known as the spindle assembly checkpoint). During mitosis, Mad1 recruits Mad2 to unattached kinetochores, where Mad2
Timo E F Kuijt et al.
Chromosoma, 123(5), 471-480 (2014-04-04)
Fidelity of chromosome segregation is monitored by the spindle assembly checkpoint (SAC). Key components of the SAC include MAD1, MAD2, BUB1, BUB3, BUBR1, and MPS1. These proteins accumulate on kinetochores in early prometaphase but are displaced when chromosomes attach to
Garry G Sedgwick et al.
mAbs, 8(4), 689-697 (2016-03-18)
The spindle assembly checkpoint (SAC) ensures accurate chromosome segregation during mitosis by delaying the activation of the anaphase-promoting complex/cyclosome (APC/C) in response to unattached kinetochores. The Mad2 protein is essential for a functional checkpoint because it binds directly to Cdc20
Yuqing Zhang et al.
Communications biology, 7(1), 164-164 (2024-02-10)
Accurate mitosis is coordinated by the spindle assembly checkpoint (SAC) through the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex or cyclosome (APC/C). As an essential regulator, Cdc20 promotes mitotic exit through activating APC/C and monitors kinetochore-microtubule attachment through
Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex.
Gang Zhang et al.
The EMBO journal, 38(7) (2019-02-21)
Kinetochore localized Mad1 is essential for generating a "wait anaphase" signal during mitosis, hereby ensuring accurate chromosome segregation. Inconsistent models for the function and quantitative contribution of the two mammalian Mad1 kinetochore receptors: Bub1 and the Rod-Zw10-Zwilch (RZZ) complex exist.
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