跳转至内容
Merck
CN

M8042

Eagle最低必需培养基

Alpha Modification, with nucleosides and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

MEM

登录 查看组织和合同定价。

选择尺寸

关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

Eagle最低必需培养基, Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

正在寻找类似产品? 访问 产品对比指南

Application

最小必需培养基 Eagle 已被用于间充质干细胞、脂肪干细胞 和成骨细胞的分离。
最小必需培养基 Eagle 用于原代成骨细胞培养 和从脐血中分离多形核白细胞 (PMN)。

Biochem/physiol Actions

MEM 通常在 15 psi 下用 0.5% 细菌蛋白胨高压灭菌 20 min，冷却，然后用 NaHCO3 中和，始终支持细胞生长良好。当 MEM 和细菌蛋白胨分别进行高压灭菌时，获得了相似的结果。细胞最初以单层生长，随后成为固定悬浮液。

General description

Harry Eagle 开发的最小必需培养基 (MEM) 是所有合成细胞培养基中应用最广泛的一种。这是所提供 MEM 制剂的最丰富变体。它含有全部 21 种正常氨基酸，有些浓度增加。此外，它还含有 5 种额外的维生素。当补充胎牛血清 (FBS) 时，通常用于细胞遗传学分析。

Preparation Note

补充 0.292 g/L L -谷氨酰胺

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动植物来源培养基
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBL9393
RNBL0710
RNBK3712
RNBK2051
RNBJ4241

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone
Schmidt E and Eriksson M
BMC Research Notes, 4(1), 282-282 (2011)
L Keay
Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01)
The growth of L-60TM cells (a suspension culture adapted L-cell) on media composed of MEM (minimum essential medium (Eagle)) and bactopeptone autoclaved together or separately under a variety of conditions has veen determined. It has been found that MEM autoclaved
Isolation of adult stem cells and their differentiation to Schwann cells
Progenitor Cells, 47-57 (2012)
Eva Schmidt et al.
BMC research notes, 4, 282-282 (2011-08-13)
The tetracycline-controlled transactivator system is a powerful tool to control gene expression in vitro and to generate consistent and conditional transgenic in vivo model organisms. It has been widely used to study gene function and to explore pathological mechanisms involved
A Maharaj et al.
The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
Deficiency in Sphingosine-1-phosphate lyase (S1P lyase) is associated with a multi-systemic disorder incorporating primary adrenal insufficiency (PAI), steroid resistant nephrotic syndrome and neurological dysfunction. Accumulation of sphingolipid intermediates, as seen with loss of function mutations in SGPL1, has been implicated
查看所有相关文献

商品

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持