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安全信息

M8042

Sigma-Aldrich

Eagle最低必需培养基

Alpha Modification, with nucleosides and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

MEM

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

产品名称

Eagle最低必需培养基, Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

质量水平

400

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

Harry Eagle 开发的最小必需培养基 (MEM) 是所有合成细胞培养基中应用最广泛的一种。这是所提供 MEM 制剂的最丰富变体。它含有全部 21 种正常氨基酸，有些浓度增加。此外，它还含有 5 种额外的维生素。当补充胎牛血清 (FBS) 时，通常用于细胞遗传学分析。

应用

最小必需培养基 Eagle 已被用于间充质干细胞、脂肪干细胞 和成骨细胞的分离。
最小必需培养基 Eagle 用于原代成骨细胞培养 和从脐血中分离多形核白细胞 (PMN)。

生化/生理作用

MEM 通常在 15 psi 下用 0.5% 细菌蛋白胨高压灭菌 20 min，冷却，然后用 NaHCO3 中和，始终支持细胞生长良好。当 MEM 和细菌蛋白胨分别进行高压灭菌时，获得了相似的结果。细胞最初以单层生长，随后成为固定悬浮液。

重悬

补充 0.292 g/L L -谷氨酰胺

补充剂

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价格

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说明
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储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
RNBL9393
RNBL0710
RNBK3712
RNBK2051
RNBJ4241

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A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone
Schmidt E and Eriksson M
BMC Research Notes, 4(1), 282-282 (2011)
Isolation of adult stem cells and their differentiation to Schwann cells
Progenitor Cells, 47-57 (2012)
L Keay
Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01)
The growth of L-60TM cells (a suspension culture adapted L-cell) on media composed of MEM (minimum essential medium (Eagle)) and bactopeptone autoclaved together or separately under a variety of conditions has veen determined. It has been found that MEM autoclaved
Eva Schmidt et al.
BMC research notes, 4, 282-282 (2011-08-13)
The tetracycline-controlled transactivator system is a powerful tool to control gene expression in vitro and to generate consistent and conditional transgenic in vivo model organisms. It has been widely used to study gene function and to explore pathological mechanisms involved
A Maharaj et al.
The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
Deficiency in Sphingosine-1-phosphate lyase (S1P lyase) is associated with a multi-systemic disorder incorporating primary adrenal insufficiency (PAI), steroid resistant nephrotic syndrome and neurological dysfunction. Accumulation of sphingolipid intermediates, as seen with loss of function mutations in SGPL1, has been implicated
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