生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~200 kDa
种属反应性
rat, human, canine
技术
western blot (chemiluminescent): 1:200 using whole cell extracts of cultured dog MDCK kidney cells, cultured rat NRK cells, and cultured human Jurkat cells.
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
rat ... Myh10(79433)
一般描述
Myosin IIB (nonmuscle) is a relatively abundant, widespread two headed myosin composed of an N-terminal motor domain, a light chain binding neck region, a coiled-coil region, and a nonhelical C-terminal domain. It forms a heterohexamer composed of a pair of heavy chains and two pairs of light chains. Myosin IIB is expressed in embryonic cardiac myocytes.
特异性
Anti-Myosin IIB, non-muscle antibody is specific for the heavy chain of human myosin IIB (nonmuscle). The antibody also reacts with myosin in dogs and rats.
免疫原
Synthetic peptide corresponding to amino acid residues 1965-1976 of the heavy chain of human myosin IIB (nonmuscle) conjugated to KLH. This sequence is identical in rabbit and differs by one and two amino acids from the respective bovine and rat sequences.
应用
Anti-Myosin IIB, non-muscle antibody produced in rabbit has been used in:
- immunoblotting
- stimulated emission depletion (STED) nanoscopy
- immunofluorescence
生化/生理作用
Myosin IIB plays a key role in the COS-7 cell cytokinesis and is need for the initiation of ring constriction.
Myosins are actin-dependent motors that have 15 classes based on their phylogenetics . Myosin IIb regulates memory and synaptic plasticity functions. Nonmuscle myosin IIb has been implicated in fibroblast movement and erythroblast enucleation .
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Actin waves do not boost neurite outgrowth in the early stages of neuron maturation
Mortal S, et al.
Frontiers in Cellular Neuroscience, 11(12), 402-402 (2017)
Nonmuscle myosin II exerts tension but does not translocate actin in vertebrate cytokinesis
Ma X, et al.
Proceedings of the National Academy of Sciences of the USA, 109(12), 4509-4514 (2012)
Katherine Pfister et al.
Development (Cambridge, England), 143(4), 715-727 (2016-02-18)
Vertebrate embryos undergo dramatic shape changes at gastrulation that require locally produced and anisotropically applied forces, yet how these forces are produced and transmitted across tissues remains unclear. We show that depletion of myosin regulatory light chain (RLC) levels in
Myosin II recruitment during cytokinesis independent of centralspindlin-mediated phosphorylation
Beach JR and Egelhoff TT
Test, 284(40), 27377-27383 (2009)
The role of pulmonary vascular contractile protein expression in pulmonary arterial hypertension
Konik EA, et al.
Journal of Molecular and Cellular Cardiology, 65(12), 147-155 (2013)
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