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生物来源
mouse
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
DCS-141, monoclonal
分子量
antigen ~80 kDa
包含
15 mM sodium azide
种属反应性
human, Xenopus
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:2,000 using HeLa cell nuclear preparation
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MCM7(4176)
一般描述
Minichromosome maintenance complex component 7 (MCM7) is part of the pre-replication complex.
Monoclonal Anti-MCM7 (CDC47) (mouse IgG1 isotype) is derived from the DCS-141 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The MCM7 gene is mapped to human chromosome 7q22.1.
特异性
Recognizes human Mcm7 and also a weaker lower molecular weight doublet.
免疫原
recombinant human Mcm7 (Cdc47) protein
应用
Monoclonal Anti-Mcm7 antibody produced in mouse has been used in:
- immunoblotting
- immunocytochemistry
- immunoprecipitation
生化/生理作用
MCM7 levels do not vary with cell cycle, but the expression and nascent synthesis of MCM7 occurs late in the cell cycle, coinciding with mitosis. MCM7 immunolocalization may be used as an index of cell proliferation in tissue sections. Monoclonal antibody reacting specifically with MCM7 is also a useful tool in the investigation of the molecular mechanisms behind DNA replication and its restriction to S phase. MCM7 may play a role in normal and neoplastic cell growth. MCM7 favors hepatocellular carcinoma progression.
Minichromosome maintenance complex component 7 (MCM7) has been shown to possess DNA helicase activity and is crucial for initiating replication.
目标描述
Mcm7 (Cdc47) belongs to the minichromosome maintenance family.
外形
The product is provided as ascites fluid with 15 mM sodium azide (see MSDS) as a preservative.
储存及稳定性
For continuous use, store at 2-8 degree C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
历史批次信息供参考:
Kai Qu et al.
Cell death & disease, 8(2), e2603-e2603 (2017-02-10)
DNA replication is a central procedure of cell proliferation, whereas aberrant DNA replication is indicated to be a driving force of oncogenesis. Minichromosome maintenance complex component 7 (MCM7) plays an essential role in initiating DNA replication. To investigate the potential
Takatsugu Kan et al.
Gastroenterology, 136(5), 1689-1700 (2009-05-08)
Barrett's esophagus (BE) is a highly premalignant disease that predisposes to the development of esophageal adenocarcinoma (EAC); however, the involvement of microRNAs (miRs) in BE-EAC carcinogenic progression is not known. Esophageal cultured cells (HEEpiC, QhTRT, ChTRT, GihTRT, and OE-33) and
Katerina Vrzalikova et al.
Pathogens (Basel, Switzerland), 1(2), 83-101 (2012-01-01)
B-lymphocyte-induced maturation protein 1 (BLIMP1) exists as two major isoforms, α and β, which arise from alternate promoters. Inactivation of the full length BLIMP1α isoform is thought to contribute to B cell lymphomagenesis by blocking post-germinal centre (GC) B cell
B K Tye
Annual review of biochemistry, 68, 649-686 (2000-06-29)
The MCM proteins are essential replication initiation factors originally identified as proteins required for minichromosome maintenance in Saccharomyces cerevisiae. The best known among them are a family of six structurally related proteins, MCM2-7, which are evolutionally conserved in all eukaryotes.
Masatoshi Takagi et al.
The FEBS journal, 275(24), 6109-6122 (2008-11-20)
Sister chromatid cohesion is important for the correct alignment and segregation of chromosomes during cell division. Although the cohesin complex has been shown to play a physical role in holding sister chromatids together, its loading onto chromatin is not sufficient
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