所有图片(4)
About This Item
经验公式（希尔记法）:
C16H18O8
CAS号:
分子量:
338.31
Beilstein:
94674
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.32
质量水平
检测方案
≥98% (TLC)
形式
powder
溶解性
water: 50 mg/mL, clear, colorless to very faintly yellow
荧光
λex 317 nm; λem 374 nm (pH 9.1)
λex 360 nm; λem 449 nm (Reaction product)
储存温度
−20°C
SMILES字符串
CC1=CC(=O)Oc2cc(O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)ccc12
InChI
1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16+/m1/s1
InChI key
YUDPTGPSBJVHCN-CHUNWDLHSA-N
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应用
4-甲基伞形基-α-D-吡喃半乳糖苷已用作人工底物，测定 α-半乳糖苷酶-A 活性。
生化/生理作用
4-甲基伞形基α-D-吡喃半乳糖苷是 α-半乳糖苷酶-A 的荧光底物。这种酶将底物转化为蓝色荧光产物甲基伞形花。通过分光光度法测定荧光强度。
制备说明
4-甲基伞形酮α-D-吡喃半乳糖苷可溶于水(50 mg/ml)，形成无色至淡黄色的澄清溶液（加热处理后）。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
Nathaniel G Hentz et al.
Journal of laboratory automation, 19(2), 153-162 (2013-09-14)
This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays
Zhen-Dan Shi et al.
Analytical and bioanalytical chemistry, 394(7), 1903-1909 (2009-06-13)
Alpha-galactosidase A hydrolyzes the terminal alpha-galactosyl moieties from glycolipids and glycoproteins in lysosomes. Mutations in alpha-galactosidase cause lysosomal accumulation of the glycosphingolipid, globotriaosylceramide, which leads to Fabry disease. Small-molecule chaperones that bind to mutant enzyme proteins and correct their misfolding
Eugènia Ruiz-Cánovas et al.
Colloids and surfaces. B, Biointerfaces, 208, 112123-112123 (2021-09-28)
The capability of HeLa cells to internalize large spherical microparticles has been evaluated by using inorganic, magnetic microparticles of 1 and 2.8 µm of diameter. In both absence but especially under the action of a magnet, both types of particles were
Tali Kizhner et al.
Molecular genetics and metabolism, 114(2), 259-267 (2014-08-27)
Fabry disease is an X-linked recessive disorder caused by the loss of function of the lysosomal enzyme α-Galactosidase-A. Although two enzyme replacement therapies (ERTs) are commercially available, they may not effectively reverse some of the Fabry pathology. PRX-102 is a
Júlia Szucs et al.
The Analyst, 134(8), 1601-1607 (2010-05-08)
An enzyme-linked immunosorbent assay (ELISA) for prostate specific antigen (PSA) detection in human serum was developed based on the potentiometric detection of 6,8-difluoro-4-methylumbelliferone (DiFMU). The assays were carried out in anti-human PSA capture antibody modified microtiter plates (150 microL volume).
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