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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
Men-8, monoclonal
形式
buffered aqueous solution
分子量
antigen ~75 kDa
种属反应性
mouse, human, rat, monkey
包装
antibody small pack of 25 μL
技术
immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/test using nuclear extract of cultured human acute T cell leukemia Jurkat cells or MCF7 cells
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MECP2(4204)
mouse ... Mecp2(17257)
rat ... Mecp2(29386)
相关类别
一般描述
Methyl-CpG binding protein 2 (MeCP2) possesses a methyl-CpG-binding domain (MBD) and a transcriptional repression domain.
Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Men-8 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminus of human MeCP2.
免疫原
synthetic peptide corresponding to the N-terminus (amino acids 15-30) of human MeCP2.
应用
Monoclonal Anti-MeCP2 antibody has been used:
- in western blotting
- in immunoblotting
- in flow cytometry
- in immunohistofluorescence
- in immunocytochemistry
生化/生理作用
MeCP2 (methyl-CpG binding protein 2) silences transcription by recruiting the histone deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes.
Methyl-CpG binding protein 2 (MeCP2) has been shown be crucial for brain development. It part of the methyl-CpG-binding proteins, which are involved in repression of gene expression by binding to methylated DNA. Mutations in the gene encoding MeCP2 have been associated with Rett syndrome (RTT).
外形
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
A codon-optimized Mecp2 transgene corrects breathing deficits and improves survival in a mouse model of Rett syndrome
Matagne V, et al.
Neurobiology of Disease, 99, 1-11 (2017)
Shikun He et al.
Investigative ophthalmology & visual science, 56(9), 5579-5589 (2015-08-26)
The purpose of this study was to evaluate expression of methyl-CpG-binding protein 2 (MeCP2) in epiretinal membranes from patients with proliferative vitreoretinopathy (PVR) and to investigate effects of inhibition of MeCP2 and DNA methylation on transforming growth factor (TGF)-β-induced retinal
Gang Meng et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(4), 3009-3015 (2015-01-24)
Methyl-CpG-binding protein 2 (MeCP2) is essential in human brain development and has been linked to several cancer types and neuro-developmental disorders. This study aims to screen the MeCP2 related differentially expressed genes and discover the therapeutic targets for osteosarcoma. CCK8
Smitha Sripathy et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(7), 1619-1624 (2017-02-02)
Rett syndrome (RS) is a debilitating neurological disorder affecting mostly girls with heterozygous mutations in the gene encoding the methyl-CpG-binding protein MeCP2 on the X chromosome. Because restoration of MeCP2 expression in a mouse model reverses neurologic deficits in adult
Radically truncated MeCP2 rescues Rett syndrome-like neurological defects
Tillotson R, et al.
Nature, 550(7676), 398-398 (2017)
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