M7318
Anti-MBD2a,b (RA-18) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
别名:
Anti-DMTase, Anti-NY-CO-41
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
indirect immunofluorescence
microarray
western blot
microarray
western blot
Species reactivity:
mouse, human
Citations:
15
Technique(s):
indirect immunofluorescence: 6-8 μg/mL using NIH3T3 cells
microarray: suitable
western blot: 0.5-1.0 μg/mL using nuclear extracts of HeLa cells
microarray: suitable
western blot: 0.5-1.0 μg/mL using nuclear extracts of HeLa cells
Uniprot accession no.:
产品名称
Anti-MBD2a,b (RA-18) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse, human
technique(s)
indirect immunofluorescence: 6-8 μg/mL using NIH3T3 cells
microarray: suitable
western blot: 0.5-1.0 μg/mL using nuclear extracts of HeLa cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MBD2(8932)
mouse ... Mbd2(17191)
Application
Anti-MBD2a,b (RA-18) antibody produced in rabbit has been used:
- for chromatin immunoprecipitation and western blotting
- immunofluorescence
- immunoblotting
Biochem/physiol Actions
MBD2 binds to methylated DNA in vitro or in vivo, suggesting that MBD2 targets the MeCP1 complex to methylated DNA. MBD2a interacts with RNA Helicase A (RHA). MBD2 protein appear to protect against some cancers.
Methyl-CpG binding domain protein 2 (MBD2a) acts as a repressor during transcription.
The antibody recognizes MBD2a (~45 kDa) and MBD2b (~29 kDa). In immunoblotting, an unidentified band at ~43 kDa may also be detected.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-MBD2a,b (RA-18) is developed in rabbit using as immunogen a synthetic peptide corresponding human MBD2a conjugated to KLH. MBD2 consists of two isoforms, MBD2a and MBD2b, which are generated from a single gene; MBD2a is a 414 amino acids protein, whereas MBD2b lacks the 152 amino acids N-terminal extension. MBD2a is a component of the MeCP1 corepressor complex, which is a 400-800 kDa complex containing as components MBD2, Mi-2, MTA2, MBD3, and HDAC1/2.
The gene encoding methyl-CpG binding domain protein 2 (MBD2a) is localized on chromosome 18.
Immunogen
synthetic peptide corresponding to amino acids 294-411 of human MBD2a conjugated to KLH via an N-terminal added lysine residue. This sequence is conserved in MBD2a and MBD2b of human origin, and is not found in MBD3.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Methyl-CpG-binding protein MBD2 plays a key role in maintenance and spread of DNA methylation at CpG islands and shores in cancer
Stirzaker C, et al.
Oncogene, 36(10), 1328-1328 (2017)
Epigenetic silencing of serine protease HTRA1 drives polyploidy
Schmidt N, et al.
BMC Cancer, 16(1), 399-399 (2016)
Pablo Gómez-Del Arco et al.
Cell metabolism, 23(5), 881-892 (2016-05-12)
Heart muscle maintains blood circulation, while skeletal muscle powers skeletal movement. Despite having similar myofibrilar sarcomeric structures, these striated muscles differentially express specific sarcomere components to meet their distinct contractile requirements. The mechanism responsible is still unclear. We show here
Methyl-CpG binding proteins in the nervous system
Guoping F and Hutnick L
Cell research, 15(4), 255-255 (2005)
Antithetic effects of MBD2a on gene regulation
Fujita H, et al.
Molecular and cellular biology, 23(8), 2645-2657 (2003)
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