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Merck
CN

M7279

Sigma-Aldrich

N,N′-亚甲基双丙烯酰胺

powder, for molecular biology, suitable for electrophoresis, ≥99.5%

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别名:
BIS
线性分子式:
(CH2=CHCONH)2CH2
CAS号:
分子量:
154.17
Beilstein:
1706297
EC 号:
MDL编号:
UNSPSC代码:
41105319
PubChem化学物质编号:
NACRES:
NB.22

等级

for molecular biology

质量水平

检测方案

≥99.5%

形式

powder

技术

electrophoresis: suitable

杂质

≤0.05% Free carboxylic acid (T)
≤50 mg/kg Total sulfur (as SO4 ) (ICP)

电导率

≤10 μmho (2% solution)

痕量阴离子

chloride (Cl-): ≤50 mg/kg

痕量阳离子

Ca: ≤10 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤5 ppm
Cu: ≤5 ppm
Fe: ≤5 ppm
K: ≤50 ppm
Mg: ≤5 ppm
Mn: ≤5 ppm
Na: ≤50 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Zn: ≤5 ppm

吸光度

≤0.2 at 290 at 1%

异质活性

DNase and RNase, none detected

SMILES字符串

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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一般描述

N,N′-亚甲基双丙烯酰胺通常简称为bis。它可在由丙烯酰胺单体链组成的聚丙烯酰胺凝胶制备中用作交联剂。N,N′-亚甲基双丙烯酰胺和丙烯酰胺的浓度决定了凝胶的孔径大小。较高浓度的Bis形成孔径较小的刚性凝胶,因为交联越多聚合程度越高,反之亦然。

应用

N,N′-亚甲基双丙烯酰胺已用于:
  • 作为单体溶液中的交联剂,制备N,N-二甲基丙烯酰胺(DMAAm)型冷冻凝胶和丙烯酰胺(AAm)型冷冻凝胶基质
  • 作为交联剂制备高强度水凝胶网络
  • 在丙烯酰胺/双丙烯酰胺储备原液的制备中用于制备聚丙烯酰胺凝胶

与丙烯酰胺混合以制备聚丙烯酰胺凝胶,用于蛋白质和核酸电泳。

象形图

Skull and crossbonesHealth hazard

警示用语:

Danger

危险分类

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Carc. 1B - Muta. 1B - Repr. 2 - STOT RE 1 Oral

靶器官

Peripheral nervous system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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