推荐产品
质量水平
无菌性
sterile-filtered
表单
liquid
储存条件
protect from light
技术
cell culture | embryo: suitable
杂质
endotoxin, tested
组分
HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
运输
ambient
储存温度
2-8°C
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应用
推荐用于室温下操作小鼠胚胎。M2 和 M16 培养基是植入前期胚胎体外培养的常用培养基。它是一种改良的Krebs-Ringer碳酸氢盐溶液，与Whitten′s培养基非常相似。M16含有丙酮酸和乳酸作为能量来源，因为植入前胚胎不能有效利用葡萄糖。
M2培养基是对M16的进一步改良，用HEPES缓冲液替代一些碳酸氢盐。M2可用于在CO2培养箱外长时间收集和处理胚胎。
M2培养基是对M16的进一步改良，用HEPES缓冲液替代一些碳酸氢盐。M2可用于在CO2培养箱外长时间收集和处理胚胎。
质量
溶液中的沉淀不影响本品性能。
重悬
添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。
分析说明
该产品经过测试，能够支持单细胞小鼠胚胎发育成扩大的胚泡。对胚泡发育的最低要求是 80％。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Maryse Lessard et al.
Scientific reports, 9(1), 13829-13829 (2019-09-27)
The paternal environment is thought to influence sperm quality and future progeny may also be impacted. We hypothesized that prenatal exposure to environmentally-relevant contaminants impairs male reproduction, altering embryo gene expression over multiple generations. Folic acid (FA) can improve sperm
Hongzheng Sun et al.
Biology of reproduction, 100(3), 601-617 (2018-09-29)
Methionine adenosyltransferase II (MAT2A) is essential to the synthesis of S-adenosylmethionine, a major methyl donor, from L-methionine and ATP. Upon fertilization, zygotic genome activation (ZGA) marks the period that transforms the genome from transcriptional quiescence to robust transcriptional activity. During
Yixin Xu et al.
Gene expression patterns : GEP, 25-26, 46-58 (2017-06-07)
Cumulus cells (CCs) are considered as an important source to predict oocyte quality. Despite numerous candidate genes in CCs have been identified for embryonic developmental competence, the results are inconsistent. The next generation RNA-sequencing was used to investigate the transcriptomic
Sarah E M Stephenson et al.
Scientific reports, 8(1), 7528-7528 (2018-05-16)
Mutations in PARK2 (parkin) can result in Parkinson's disease (PD). Parkin shares a bidirectional promoter with parkin coregulated gene (PACRG) and the transcriptional start sites are separated by only ~200 bp. Bidirectionally regulated genes have been shown to function in common
Yi-Wen Xu et al.
Journal of cell science, 130(19), 3297-3307 (2017-08-19)
Precise regulation of DNA replication and genome integrity is crucial for gametogenesis and early embryogenesis. Cullin ring-finger ubiquitin ligase 4 (CRL4) has multiple functions in the maintenance of germ cell survival, oocyte meiotic maturation, and maternal-zygotic transition in mammals. DDB1-cullin-4-associated
商品
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
实验方案
使用CRISPR/Cas9系统生成正确的转基因小鼠需要充分理解正在测试的假设。了解如何创建成功的小鼠模型项目。
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