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表单
lyophilized powder
比活
≥50 units/mg solid
储存温度
−20°C
一般描述
Malic dehydrogenase from Thermus flavus is stable and active at 90 ° C.
应用
Malic dehydrogenase has been used in a study to assess electron transport chain activity in mitochondria from human skeletal muscle. It has also been used in a study to investigate activities of enzymes and ammonia in serum of rats with fluoride hyperglycemia.
单位定义
One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.
外形
Contains dextran
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
C A Kelly et al.
Biochemistry, 32(15), 3913-3922 (1993-04-20)
A binary complex of malate dehydrogenase from the thermophilic bacterium Thermus flavus (tMDH) with NADH has been crystallized from poly(ethylene glycol) 3500, pH 8.5, yielding diffraction-quality crystals in space group P2(1)2(1)2(1). The structure was solved at 1.9-A resolution using molecular
Ewa Grucka-Mamczar et al.
Annales Academiae Medicae Stetinensis, 50 Suppl 1, 36-41 (2006-08-09)
The study was done in 30 one-month-old Wistar FL rats divided into one control and two study groups of ten animals each. Hyperglycemia was induced with sodium fluoride in water at a concentration of 50 or 100 mg/L during four
G Ferreira et al.
Journal of dairy science, 90(3), 1460-1466 (2007-02-14)
Biotin is a cofactor of the gluconeogenic enzymes pyruvate carboxylase (PC) and propionyl-coenzyme A carboxylase (PCC). We hypothesized that biotin supplementation increases the activity and gene expression of PC and PCC and the gene expression of phosphoenol-pyruvate carboxykinase (PEPCK) in
Vladimir B Ritov et al.
Analytical biochemistry, 333(1), 27-38 (2004-09-08)
This study addresses an application of pyridine nucleotide enzymatic analyses to evaluate the activity of the mitochondrial electron transport chain (reduced nicotinamide adenine dinucleotide (NADH) oxidase) and Complexes I and II in samples of human muscle as small as approximately
Allan E Strand et al.
PloS one, 7(9), e45138-e45138 (2012-10-11)
A common geographical pattern of genetic variation is the one-dimensional cline. Clines may be maintained by diversifying selection across a geographical gradient but can also reflect historical processes such as allopatry followed by secondary contact. To identify loci that may
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