生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
MA454, monoclonal
表单
buffered aqueous solution
分子量
antigen ~46 kDa
种属反应性
human
包装
antibody small pack of 25 μL
浓度
~2 mg/mL
技术
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 5-10 μg/mL using total cell extract of SKML37 cells
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MAGEA1(4100)
一般描述
Melanoma-associatiated antigen 1 (MAGE-1) is part of the CG (cancer-germline) group of genes and is a tumor-specific antigen. MAGE-1 is a member of the MAGE-A family. MAGE-1 gene is localized on human chromosome Xq28. Monoclonal Anti-MAGE-1 (mouse IgG1 isotype) is derived from the hybridoma MA454 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with human recombinant MAGE-1.
特异性
The antibody does not recognize MAGE-2 and MAGE-3.
免疫原
recombinant human MAGE-1.
应用
Monoclonal Anti-MAGE-1 antibody produced in mouse has been used in:
- ELISA
- immunoblotting
- immunohistochemistry
生化/生理作用
The melanoma antigen genes (MAGE) might play a key role in neural development and apoptosis. Expression of melanoma-associated antigen 1 (MAGE-1) in neoplastic cells depends on the binding of the transcription factor Ets to non-methylated cis regulatory sequences, located in its promoter. MAGE-1 expression is mediatedby the B and B′ domains of MAGE-A1 gene. Expression of MAGE-1A was induced in non MAGE-A1 expressing tumor cells once treated with demethylating reagent, 5-aza-2-(5DC). Melanoma-associatiated antigen 1 (MAGE-1) has been shown to be expressed in different types of cancers.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
The MAGE Proteins: Emerging Roles in Cell
Cycle Progression, Apoptosis, and
Neurogenetic Disease
Cycle Progression, Apoptosis, and
Neurogenetic Disease
Barker PA and Salehi A
Journal of Neuroscience Research (2002)
Madhav V Dhodapkar et al.
Cancer immunity, 3, 9-9 (2003-07-24)
Cancer/testis (CT) antigens are expressed in several malignant tumors, but not in normal tissues except for testicular germ cells. The expression of CT antigenic proteins in malignant gammopathies has not been characterized. We examined the expression of a panel of
Clinical Significance of Melanoma Antigen-Encoding Gene-1 (MAGE-1) Expression and its Correlation with Poor Prognosis in Differentiated Advanced Gastric Cancer
Ogata MDK, et al.
Annals of Surgical Oncology (2011)
Y T Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(3), 1004-1008 (1994-02-01)
The human MAGE-1 gene encodes a melanoma peptide antigen recognized by autologous cytotoxic T lymphocytes. To produce antibodies against the MAGE-1 gene product, several approaches were taken. Three oligopeptides were synthesized based on predicted MAGE-1 amino acid sequences and were
Oliver Goodyear et al.
Blood, 116(11), 1908-1918 (2010-06-10)
Epigenetic therapies, including DNA methyltransferase and histone deacetylase inhibitors, represent important new treatment modalities in hematologic malignancies, but their mechanism of action remains unknown. We reasoned that up-regulation of epigenetically silenced tumor antigens may induce an immunologically mediated antitumor response
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