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生物来源
mouse
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
Munc13-71, monoclonal
表单
buffered aqueous solution
种属反应性
rat, mouse
浓度
~2 mg/mL
技术
microarray: suitable
western blot: 1-2 μg/mL using cytosolic rat brain S1 fraction
同位素/亚型
IgG2b
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
mouse ... Unc13a(382018)
rat ... Unc13a(64829)
一般描述
Monoclonal Anti-Munc13-1 (mouse IgG2b isotype) is derived from the hybridoma Munc13-71 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of rat Munc13-1, conjugated to KLH. Protein unc-13 homolog A (Munc13-1) contains a diacylglycerol (DAG)/b phorbol ester-binding C1 domain. Munc13-1 are expression in axon is observed at various synapse populations.
Munc13/1 is a diacylglycerol receptor which is expressed in the presynaptic area.
免疫原
synthetic peptide corresponding to amino acid 10-27 of rat munc13/1, conjugated to KLH.
应用
Monoclonal Anti-Munc13/1 antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA) and immunoblotting.
生化/生理作用
Munc13/1 has a crucial role in synaptic vesicle priming. It interacts with Syntaxin 1A and helps it to bind to soluble NSF attachment protein receptor (SNARE) proteins. Thus it contributes to the stability of the SNARE complex. Deletion of Munc13/1 leads to complete arrest synaptic transmission.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Differential control of vesicle priming and short-term plasticity by Munc13 isoforms
Rosenmund C, et al.
Neuron, 33(3), 411-424 (2002)
ELKS/Voltage-dependent Ca2+ channel-beta subunit module regulates polarized Ca2+ influx in pancreatic beta cells
Ohara-Imaizumi M, et al.
Testing, 26(5), 1213-1226 (2019)
Edwin P Kwan et al.
Diabetes, 55(5), 1421-1429 (2006-04-29)
Munc13-1 is a diacylglycerol (DAG) receptor that is essential for synaptic vesicle priming. We recently showed that Munc13-1 is expressed in rodent and human islet beta-cells and that its levels are reduced in islets of type 2 diabetic humans and
Lijun Kang et al.
Cell metabolism, 3(6), 463-468 (2006-05-16)
Munc13-1 is a presynaptic protein that is essential for synaptic vesicle priming. Deletion of Munc13-1/unc13 causes total arrest of synaptic transmission due to a complete loss of fusion-competent synaptic vesicles. The requirement of Munc13-1 for large dense-core vesicles (LDCVs), however
Stefan Kalla et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(50), 13054-13066 (2006-12-15)
GFP (green fluorescent protein) fusion proteins have revolutionized research on protein dynamics at synapses. However, corresponding analyses usually involve protein expression methods that override endogenous regulatory mechanisms, and therefore cause overexpression and temporal or spatial misexpression of exogenous fusion proteins
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