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Merck
CN

M6190

Sigma-Aldrich

抗-MYOD1单克隆抗体 小鼠抗

clone 5.2F, purified immunoglobulin, buffered aqueous solution

别名:

抗-肌源性分化抗原1

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

5.2F, monoclonal

表单

buffered aqueous solution

分子量

antigen 34 kDa

种属反应性

human, rat, chicken, mouse

浓度

1.0 mg/mL

技术

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL
immunohistochemistry (frozen sections): 2-4 μg/mL
immunoprecipitation (IP): 2 μg using 1 mg protein lysate
western blot: 1 μg/mL (reacts with the ~45 kDa protein)

同位素/亚型

IgG2a

UniProt登记号

运输

wet ice

储存温度

−20°C

基因信息

human ... MYOD1(4654)
mouse ... Myod1(17927)
rat ... Myod1(337868)

一般描述

成肌分化抗原1(MYOD1)是一种在骨骼肌中表达的核蛋白。它是碱性螺旋-环-螺旋蛋白(bHLH)转录因子家族的一员。编码它的基因位于人染色体11上。

免疫原

重组小鼠 MyoD1 蛋白。

应用

小鼠中生产的单克抗-MYOD1 抗体已被用于:
  • 1:50 稀释度下进行免疫荧光染色
  • 免疫印迹
  • 1:300 稀释度下进行免疫染色

生化/生理作用

成肌分化抗原1(MYOD1)可能参与将乙酰转移酶和甲基转移酶等酶招募到人类基因组中的成肌增强子上。它参与肌肉的再生并通过激活细胞周期停滞来介导肌肉细胞分化。

外形

在含有 0.08% 叠氮化钠的磷酸盐缓冲盐水中的溶液。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Roy Blum
Journal of cellular biochemistry, 115(11), 1855-1867 (2014-06-07)
The early 1980s revelation of cis-acting genomic elements, known as transcriptional enhancers, is still regarded as one of the fundamental discoveries in the genomic field. However, only with the emergence of genome-wide techniques has the genuine biological scope of enhancers
Bolin Cai et al.
Cellular & molecular biology letters, 29(1), 9-9 (2024-01-05)
Skeletal muscle development is pivotal for animal growth and health. Recently, long noncoding RNAs (lncRNAs) were found to interact with chromatin through diverse roles. However, little is known about how lncRNAs act as chromatin-associated RNAs to regulate skeletal muscle development.
Shujie Chen et al.
Bio-protocol, 9(14), e3313-e3313 (2019-07-20)
Myofiber isolation followed with ex vivo culture could recapitulate and visualize satellite cells (SCs) activation, proliferation, and differentiation. This approach could be taken to understand the physiology of satellite cells and the molecular mechanism of regulatory factors, in terms of
Jennifer A Tinklenberg et al.
Journal of neuropathology and experimental neurology, 78(2), 130-139 (2019-01-01)
Mutations in at least 12 genes are responsible for a group of congenital skeletal muscle diseases known as nemaline myopathies (NMs). NMs are associated with a range of clinical symptoms and pathological changes often including the presence of cytoplasmic rod-like
Ramesh P Arasaradnam et al.
BMC research notes, 5, 240-240 (2012-05-18)
DNA methylation of promoter-associated CpG islands of certain genes may play a role in the development of colorectal cancer. The MYOD-1 gene which is a muscle differentiation gene has been showed to be significantly methylated in colorectal cancer which, is

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