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生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
种属反应性
rat
技术
microarray: suitable
western blot: 1:500 using rat brain extracts
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MAGI1(9223)
mouse ... Magi1(14924)
rat ... Cnksr3(308113)
一般描述
The MAGUK (Membrane Associated Guanylate kinase) family of proteins localize to regions of cell-cell contact, such as tight junctions in epithelial cells and synaptic junctions in neurons. In epithelia, membrane associated guanylate kinase, WW And PDZ domain containing 1 (MAGI-1) is localized at tight junctions and is expressed in three isoforms (MAGI-1a, MAGI-1b, and MAGI-1c). MAGI-1 was first identified in mouse as a protein interacting with K-RasB (Ras-like protein rasB).
特异性
Recognizes rat MAGI-1 by immunoblotting (approx. 170, 130, and 120 kDa) and does not cross react with MAGI-2 or MAGI-3.
免疫原
synthetic peptide corresponding to amino acids 282-296 of MAGI-1 with a carboxy terminal added cysteine residue conjugated to maleimide-activated KLH. The sequence is conserved in human and mouse.
应用
Anti-MAGI-1 antibody produced in rabbit has been used in
- immunoblotting
- western blotting
- immunostaining
生化/生理作用
Membrane associated guanylate kinase, WW And PDZ domain containing 1 (MAGI-1) interacts with β-catenin. Neuroepithelial cell-transforming gene 1(nNET1) and actin binding proteins act as binding partners of MAGI-1. Interestingly, MAGI-1c is present in nucleus, suggesting that MAGI-1 may participate in the transmission of regulatory signals from the cell surface to the nucleus.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
MAGI-1: A Widely Expressed, Alternatively Spliced Tight Junction Protein
Laura RP, et al.
Experimental Cell Research, 275(2), 155-170 (2002)
Shuqin Jia et al.
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, 29(1), 25-35 (2017-04-05)
To explore the association of membrane-associated guanylate kinase inverted 1 (MAGI1) with gastric cancer (GC) and the related molecular mechanisms. The reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) were utilized to measure the MAGI1 expression level in GC tissues.
MAGI-1: A Widely Expressed, Alternatively Spliced Tight Junction Protein
Dobrosotskaya IY, et al.
Biochemical and Biophysical Research Communications, 270(3), 903-909 (2000)
Jun-Ichi Abe et al.
JCI insight, 4(7) (2019-04-05)
The possible association between the membrane-associated guanylate kinase with inverted domain structure-1 (MAGI1) and inflammation has been suggested, but the molecular mechanisms underlying this link, especially during atherogenesis, remain unclear. In endothelial cells (ECs) exposed to disturbed flow (d-flow), p90
Peter Muench et al.
Virology, 387(2), 380-387 (2009-03-17)
There is compelling evidence that high-risk human papillomaviruses (HPV) can cause cervical cancer. Strikingly, HPV16 and 18 account for approximately 70% of all cervical cancers, whereas phylogenetically related types are found at much lower frequencies. Most likely, differences in the
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