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M5650

Eagle最低必需培养基

With non-essential amino acids, Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

MEM

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
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产品名称

Eagle最低必需培养基, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

General description

Eagle最低必需培养基(MEM)是Harry Eagle开发的人工合成细胞培养基。培养基含有较高浓度的氨基酸，因此比较接近哺乳动物培养细胞的蛋白质成分。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。通过选择性去除钙成分后，该配方可进一步改良为适合悬浮细胞培养。MEM可用于各种细胞的单层培养。

Application

Eagle最低必需培养基已用于培养：
  • HeLa细胞
  • Madin-Darby犬肾细胞 (MDCK)
  • 软骨肉瘤细胞系

此培养基在血清减量条件下适合贴壁细胞生长，也可作为无血清培养基开发的基础配方。

Preparation Note

添加 0.292g/L L-谷氨酰胺。

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存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBL9243
RNBL6714
RNBL5468
RNBL5640
RNBL4495

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商品

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Junjie Jing et al.
International journal of clinical and experimental medicine, 8(8), 13127-13135 (2015-11-10)
Tectonic family member 1 (TCTN1) encodes a member of the tectonic family which are evolutionarily conserved secreted and transmembrane proteins, involving in a diverse variety of developmental processes. It has been demonstrated that tectonics expressed in regions that participate in
3D cell sheets formed via cell-driven buckling-delamination of patterned thin films
Oyama T, et al.
Materials & Design, 208, 109975-109975 (2021)
Bryan Tay et al.
PloS one, 14(3), e0213751-e0213751 (2019-03-12)
Voltage-gated sodium channels (NaVs) are key therapeutic targets for pain, epilepsy and cardiac arrhythmias. Here we describe the development of a no-wash fluorescent sodium influx assay suitable for high-throughput screening and characterization of novel drug leads. Addition of red-violet food
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全球贸易项目编号

货号GTIN
M5650-6X500ML04061834060178
M5650-500ML04061834060161
M5650-1L04061834060154