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安全信息

M5650

Sigma-Aldrich

Eagle最低必需培养基

With non-essential amino acids, Earle′s salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

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别名:
MEM
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

400

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

运输

ambient

储存温度

2-8°C

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一般描述

Eagle最低必需培养基(MEM)是Harry Eagle开发的人工合成细胞培养基。培养基含有较高浓度的氨基酸，因此比较接近哺乳动物培养细胞的蛋白质成分。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。通过选择性去除钙成分后，该配方可进一步改良为适合悬浮细胞培养。MEM可用于各种细胞的单层培养。

应用

Eagle最低必需培养基已用于培养：
  • HeLa细胞
  • Madin-Darby犬肾细胞 (MDCK)
  • 软骨肉瘤细胞系

此培养基在血清减量条件下适合贴壁细胞生长，也可作为无血清培养基开发的基础配方。

重悬

添加 0.292g/L L-谷氨酰胺。

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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Bryan Tay et al.
PloS one, 14(3), e0213751-e0213751 (2019-03-12)
Voltage-gated sodium channels (NaVs) are key therapeutic targets for pain, epilepsy and cardiac arrhythmias. Here we describe the development of a no-wash fluorescent sodium influx assay suitable for high-throughput screening and characterization of novel drug leads. Addition of red-violet food
Christopher G Mills et al.
Scientific reports, 7(1), 14824-14824 (2017-11-03)
We present a strategy for increasing the anatomical realism of organoids by applying asymmetric cues to mimic spatial information that is present in natural embryonic development, and demonstrate it using mouse kidney organoids. Existing methods for making kidney organoids in
Junjie Jing et al.
International journal of clinical and experimental medicine, 8(8), 13127-13135 (2015-11-10)
Tectonic family member 1 (TCTN1) encodes a member of the tectonic family which are evolutionarily conserved secreted and transmembrane proteins, involving in a diverse variety of developmental processes. It has been demonstrated that tectonics expressed in regions that participate in
3D cell sheets formed via cell-driven buckling-delamination of patterned thin films
Oyama T, et al.
Materials & Design, 208, 109975-109975 (2021)
Emanuela Profilo et al.
Biochimica et biophysica acta, 1863(6), 1422-1435 (2017-02-27)
The mitochondrial aspartate-glutamate carrier isoform 1 (AGC1) catalyzes a Ca
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商品

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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