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Merck
CN

M5310

Sigma-Aldrich

矿物油

light oil, suitable for mouse embryo cell culture, BioXtra

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75
技术:
cell culture | embryo: suitable

无菌性

sterile-filtered

质量水平

产品线

BioXtra

表单

light oil

储存条件

protect from light

技术

cell culture | embryo: suitable

折射率

n20/D 1.467 (lit.)

密度

0.82-0.88 g/mL at 25 °C

包装形式

neat

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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一般描述

采用矿物油或液体石油保护各种类型的培养物。矿物油的优点是可降低生长速率,并且几乎完全消除了培养基的水分蒸发。因此,减少了传代培养,培养物活率更高。1914年,矿物油首次用于覆盖细菌培养物。

应用

矿物油已被普遍使用并推荐用于覆盖小鼠胚胎培养物,防止其在培养过程中干燥。
矿物油适用于下列应用:
  • 在细胞活性研究中用于制备培养基或培养基补充剂
  • 通过在阵列周围添加矿物油,防止阵列蒸发
  • 用于制备微量注射针(微量注射针拉伸、锐化和装载)
  • 用于PCR设置

特点和优势

即用型无菌过滤产品采用便捷的PET瓶包装。

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

No data available

闪点(°C)

No data available

个人防护装备

Eyeshields, Gloves


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分析证书(COA)

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Mineral Oil Overlay for Conservation of Plant Tissue Cultures
Caplin SM
American Journal of Botany, 46(5), 324-329 (1959)
Slobodan Beronja et al.
Methods in molecular biology (Clifton, N.J.), 961, 351-361 (2013-01-18)
We have recently developed a method for RNAi-mediated gene function analysis in skin (Beronja et al., Nat Med 16:821-827, 2010). It employs ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity of embryonic day 9 mice, which result in
Xiyan Wang et al.
Journal of clinical laboratory analysis, 34(11), e23498-e23498 (2021-01-20)
Efficiency of preantral follicle culture in vitro is low and is dependent on species, development stage, and follicle-stimulating hormone (FSH) concentration. Here, we optimized the preantral follicle in vitro culture system in mice. The primary follicles (PM follicles, 80-100 μm
Bei Zhao et al.
Scientific reports, 7(1), 11319-11319 (2017-09-14)
A microfluidic chip with a controllable and integrated piezoelectric pump was proposed and demonstrated, where the pump was designed as a micro-actuator based on polyvinylidene fluoride (PVDF) organic piezoelectric film. In this case, the pump should integrate with the microfluidics
Hanyoup Kim et al.
Optics express, 17(1), 218-227 (2009-01-09)
We report the successful application of low-power (approximately 30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature

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Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂

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