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Merck
CN

M5310

Sigma-Aldrich

矿物油

light oil, suitable for mouse embryo cell culture, BioXtra

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CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75

无菌性

sterile-filtered

质量水平

产品线

BioXtra

形式

light oil

储存条件

protect from light

技术

cell culture | embryo: suitable

折射率

n20/D 1.467 (lit.)

密度

0.82-0.88 g/mL at 25 °C

格式

neat

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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一般描述

采用矿物油或液体石油保护各种类型的培养物。矿物油的优点是可降低生长速率,并且几乎完全消除了培养基的水分蒸发。因此,减少了传代培养,培养物活率更高。1914年,矿物油首次用于覆盖细菌培养物。

应用

矿物油已被普遍使用并推荐用于覆盖小鼠胚胎培养物,防止其在培养过程中干燥。
矿物油适用于下列应用:
  • 在细胞活性研究中用于制备培养基或培养基补充剂
  • 通过在阵列周围添加矿物油,防止阵列蒸发
  • 用于制备微量注射针(微量注射针拉伸、锐化和装载)
  • 用于PCR设置

特点和优势

即用型无菌过滤产品采用便捷的PET瓶包装。

WGK

WGK 1

闪点(°F)

No data available

闪点(°C)

No data available

个人防护装备

Eyeshields, Gloves


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Mineral Oil Overlay for Conservation of Plant Tissue Cultures
Caplin SM
American Journal of Botany, 46(5), 324-329 (1959)
Bei Zhao et al.
Scientific reports, 7(1), 11319-11319 (2017-09-14)
A microfluidic chip with a controllable and integrated piezoelectric pump was proposed and demonstrated, where the pump was designed as a micro-actuator based on polyvinylidene fluoride (PVDF) organic piezoelectric film. In this case, the pump should integrate with the microfluidics
Hanyoup Kim et al.
Optics express, 17(1), 218-227 (2009-01-09)
We report the successful application of low-power (approximately 30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature
Slobodan Beronja et al.
Methods in molecular biology (Clifton, N.J.), 961, 351-361 (2013-01-18)
We have recently developed a method for RNAi-mediated gene function analysis in skin (Beronja et al., Nat Med 16:821-827, 2010). It employs ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity of embryonic day 9 mice, which result in
Hanyoup Kim et al.
Lab on a chip, 9(9), 1230-1235 (2009-04-17)
We report high-speed real-time PCR performed on an unmodified disposable polystyrene Petri dish. The reaction cycle relies solely on an infrared laser for heating; no conventional heater is required. Nanoliter droplets of PCR mixture as water-in-oil emulsions printed in an

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Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂

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