产品名称
Monoclonal Anti-Mucin Gastric antibody produced in mouse, clone 45M1, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
45M1, monoclonal
contains
15 mM sodium azide
species reactivity
chicken, monkey, rat, rabbit, pig, hedgehog, mouse, human, feline
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using sections of human stomach
western blot: suitable using non-reducing conditions
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MUC5AC(4586)
mouse ... Muc5ac(17833)
rat ... Muc5ac(682837)
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Monoclonal Anti-Human Gastric Mucin may be used for the localization of gastric mucin using various immunochemical assays such as immunoblot, immunofluorescence, immunohistochemistry, immunostaining and immunoradiofixation.
Biochem/physiol Actions
Mucin is composed of a peptide core containing heavily glycosylated regions and nonglycosylated regions. The heavily glycosylated components of mucin consist of a mosaic of epitopes, some of which are associated with saccharide moieties and are related to blood-group antigens, and others which are M1 antigens associated with the peptidic core. M1 antigens are common to human ovarian mucinous cyst fluids and to gastric mucosa. M1 is distributed in various human tumors such as the colon, stomach, pancreas, ovary, Barrets′ esophagus, endocervix, and endometrium carcinomas.
The antibody recognizes the mucin epitope g located in the peptide core of gastric mucin (>1,000 kDa). This epitope is completely destroyed by thiol reduction (using mercaptoethanol) and partially lost following trypsin proteolysis, but is stable upon periodate oxidation. The antibody stains the surface gastric epithelium of normal human gastrointestinal tract and reacts with fetal, pre-cancerous and cancerous colonic mucosa, but not with normal colon.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Human Gastric Mucin (mouse IgG1 isotype) is derived from the 445M1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with mucin isolated from human ovarian cyst fluid. Mucin is a high M.W. (1,000 kDa) glycoprotein, expressed by mucus cells of the gastric epithelium and by goblet cells of the fetal, precancerous and cancerous colon, but not by those of the normal colon. It also appears in other epithelial tissues, which are embryologically derived from the foregut (epigastric and bronchial epithelium) and in Müller ducts (mucus cells of the endocervix and urethral epithelium near the prostatic utriculus).
Mucin has a netlike, branched structure and is a secreted protein. It belongs to the category of gel-forming mucins. The gene encoding this protein is upregulated by interleukin-1β (IL-1β) and is localized on chromosome 11p15.5.
Immunogen
mucin from human ovarian cyst fluid.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Marina Bertolin et al.
Cornea, 38(4), 469-479 (2018-06-21)
To develop autologous tissue-engineered conjunctival epithelial sheets to be used as advanced therapy medicinal products for severe ocular surface disorders involving the conjunctiva. Methods used aimed at 1) mapping the conjunctiva for identification of the stem cell location, 2) establishing
MUC5AC upstream complex repetitive region length polymorphisms are associated with susceptibility and clinical stage of gastric cancer.
Wang C
PLoS ONE, 9(6), e98327-e98327 (2014)
Optimized Protocol for Regeneration of the Conjunctival Epithelium Using the Cell Suspension Technique.
Bertolin M, et al.
Cornea, 38(4), 469-479 (2019)
Emma C Skoog et al.
PloS one, 7(5), e36378-e36378 (2012-05-09)
Helicobacter pylori colonizes the mucus niche of the gastric mucosa and is a risk factor for gastritis, ulcers and cancer. The main components of the mucus layer are heavily glycosylated mucins, to which H. pylori can adhere. Mucin glycosylation differs
Gastrointestinal cell lines form polarized epithelia with an adherent mucus layer when cultured in semi-wet interfaces with mechanical stimulation.
Navabi N, et al.
PLoS ONE, 8(7), e68761-e68761 (2013)
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